Cd3e

CAT#: CL002B

Hamster monoclonal anti-CD3 antibody, clone500-A2, Biotinylated

Product Images

Cell Source: Spleen Percentage of cells stained above control: 44.1%

Specifications

Product Data

• Clone Name: 500-A2
• Applications: FC
• Recommended Dilution: FC
• Reactivities: Mouse
• Host: Hamster
• Isotype: IgG
• Clonality: Monoclonal
• Formulation: PBS, 0.1% NaN3 and EIA grade BSA as stabilizing protein to bring the total protein concentration to 4-5 mg/ml
• Concentration: lot specific
• Purification: Liquid purified IgG
• Conjugation: Unconjugated
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
  Shelf life: one year from despatch.
• Predicted Protein Size: ~21.8 kDa
• Gene Name: CD3 antigen, epsilon polypeptide
• Database Link:
  NP_031674
  Entrez Gene 12501 Mouse
  UniProt ID P22646
• Background: T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits: CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta. These CD3 subunits are structurally related members of the immunoglobulins super family encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules. This association is mediated at least in part by a double tyrosine based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR induced growth arrest, cell survival and proliferation. The CD3 antigen is present on 68-82% of normal peripheral blood lymphocytes, 65-85% of thymocytes and Purkinje cells in the cerebellum. It is never expressed on B or NK cells. Decreased percentages of T lymphocytes may be observed in some autoimmune diseases.
• Synonyms: CD3-epsilon; FLJ18683; T3E; TCRE