Cd4 Rat Monoclonal Antibody [Clone ID: CT-CD4]

CAT#: CL004R

Cd4 rat monoclonal antibody, clone CT-CD4, PE

Product Images

Cell Source: Spleen Percentage of cells stained above control: 23.3%

Specifications

Product Data

• Clone Name: CT-CD4
• Applications: FC
• Recommended Dilution: Flow Cytometry (See Protocols).
  (Reported to be useful in Immunohistochemistry on Acetone Fixed Frozen Sections).
• Reactivities: Mouse
• Host: Rat
• Isotype: IgG2a
• Clonality: Monoclonal
• Specificity: This monoclonal antibody (mAb) recognizes Mouse CD4 (L3T4) which is expressed on the majority of thymocytes and on the MHC class II restricted subset of mature T cells including Th cells.
• Formulation: PBS containing 0.02% Sodium Azide as preservative and EIA grade BSA as a stabilizing protein to bring total protein concentration to 4-5 mg/ml.
  Label: PE
  State: Liquid purified IgG fraction.
• Concentration: 0.1 mg/ml
• Conjugation: PE
• Database Link:
  Entrez Gene 12504 Mouse
• Background: Mouse CD4 has also been reported to be present on multipotential hematopoietic stem cells, bone marrow myeloid precursors, and intrathymic precursors2,3. As a coreceptor in the TCR complex, CD4 is involved in T cell activation through interaction with MHC class II on APC's and in signal transduction via protein tyrosine kinase lck1.
• Synonyms: T-cell surface antigen T4/Leu-3
• Note: Protocol: FLOW CYTOMETRY ANALYSIS:
  1. Prepare a cell suspension in media A. For cell preparations, deplete the red blood cell population with Lympholyte®-M cell separation medium.
  2. Wash 2 times.
  3. Resuspend the cells to a concentration of 2x10⁷ cells/ml in media A. Add 50 µl of this suspension to each tube (each tube will then contain 1 x 10⁶ cells, representing 1 test).
  4. To each tube, add ~1.0 µg of this Ab per 10⁶ cells.
  5. Vortex the tubes to ensure thorough mixing of antibody and cells.
  6. Incubate the tubes for 30 minutes at 4°C. (It is recommended that the tubes are protected from light, since most fluorochromes are light sensitive.)
  7. Wash 2 times at 4°C.
  8. Resuspend the cell pellet in 50 µl ice cold media B.
  9. Transfer to suitable tubes for flow cytometric analysis containing 15 µl of propidium iodide at 0.5 mg/ml in PBS. This stains dead cells by intercalating in DNA.
  
  Media:
  A. Phosphate buffered saline (pH 7.2) + 5% normal serum of host species + Sodium Azide (100 µl of 2M Sodium Azide in 100 mls).
  B. Phosphate buffered saline (pH 7.2) + 0.5% Bovine Serum Albumin + Sodium Azide (100 µl of 2M Sodium Azide in 100 mls).
  
  Tissue Distribution by Flow Cytometry Analysis:
  Mouse Strain: BALB/c
  Cell Concentration: 1x10⁶ cells per test
  Antibody Concentration Used: 1.0 µg/10⁶ cells
  Isotypic Control: PE Rat IgG2a