Cd5 Mouse Monoclonal Antibody [Clone ID: CG16]

CAT#: CL006BX

Cd5 mouse monoclonal antibody, clone CG16, Biotin


USD 435.00

2 Weeks*

Size
    • 300 ug

Product Images

Specifications

Product Data
Clone Name CG16
Applications FC
Recommended Dilution Flow cytometry analysis (see Protocols).
Reactivities Mouse
Host Mouse
Isotype IgG2b
Clonality Monoclonal
Immunogen C3H.CE - Ly 1.2 : DS from C3H spleen.
Fusion Partner: Myeloma SP2/0 - Ag 14 (M5).
Specificity This mAb reacts with T cells from mouse strains expressing the Ly 1.2 phenotype, but does not react with lympholytes from mouse strains expressing the Ly 1.1 phenotype.
Formulation PBS, 0.02% NaN3 and EIA grade BSA as a stabilizing protein to bring total protein concentration to 4-5 mg/ml
Label: Biotin
State: Liquid purified IgG
Concentration 0.1 mg/ml
Purification Protein G Chromatography
Conjugation Biotin
Background CD5 is a 55kDa T lymphocyte single chain transmembrane glycoprotein. It is present on all mature T lymphocytes, on most thymocytes and on many T cell leukemias and lymphomas. It reacts with a subpopulation of activated B cells. CD5/Lyt1 antigen is a monomeric type I transmembrane glycoprotein expressed on thymocytes, T lymphocytes, and a subset of B lymphocytes, but not on natural killer (NK) cells. It has been identified as the major ligand of the B cell antigen CD72. The frequency of CD5+ B cells exhibits strain dependent variation, and the phenotypic, anatomical, functional, developmental, and pathological characteristics of the CD5+ B cells suggest that they may represent a distinct lineage, known as B1 cells. Binding of CD5 on the T cell surface can augment alloantigen or mitogen induced lymphocyte proliferation and induces increased cytosolic free calcium, IL2 secretion, and IL2R expression. It has been proposed that CD5 negatively regulates signal transduction mediated by the T cell and B cell receptors.
Synonyms CD5, LEU1
Note Protocol: FLOW CYTOMETRY ANALYSIS:

Method:
1. Prepare a cell suspension in media A. For cell preparations, deplete the red blood cell population with Lympholyte®-M cell separation medium.
2. Wash 2 times.
3. Resuspend the cells to a concentration of 2x10e7 cells/ml in media A. Add 50 µl of this suspension to each tube (each tube will then contain 1 x 10e6 cells, representing 1 test).
4. To each tube, add 0.1 - 0.2 µg* of this Ab per 10e6 cells.
5. Vortex the tubes to ensure thorough mixing of antibody and cells.
6. Incubate the tubes for 30 minutes at 4°C.
7. Wash 2 times at 4°C.
8. Add 100 µl of secondary antibody (Streptavidin-FITC) at a 1:500 dilution.
9. Incubate tubes at 4°C for 30 - 60 minutes.
10. Wash 2 times at 4°C.
11. Resuspend the cell pellet in 50 µl ice cold media B.
12. Transfer to suitable tubes for flow cytometric analysis containing 15 µl of propidium iodide at 0.5 mg/ml in PBS. This stains dead cells by intercalating in DNA.

Media:
A. Phosphate buffered saline (pH 7.2) + 5% normal serum of host species + sodium azide (100 µl of 2M sodium azide in 100 mls).
B. Phosphate buffered saline (pH 7.2) + 0.5% Bovine serum albumin + sodium azide (100 µl of 2M sodium azide in 100 mls).

Results - Tissue Distribution
Mouse Strain: BALB/c
Cell Concentration: 1x10e6 cells per tests
Antibody Concentration Used: 0.1 µg/10e6 cells
Isotypic Control: Biotin Mouse IgG2b,k

Results - Strain Distribution
Cell Concentration: 1x10e6 cells per tests
Antibody Concentration Used: 0.1 µg/10e6 cells
Strains Tested: BALB/C, C3H/He, CBA/J, AKR, ATH
Positive: BALB/C, AKR, ATH
Negative: C3H/He, CBA/J
Reference Data

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.