Cd8a Mouse Monoclonal Antibody [Clone ID: 49-31.1]

CAT#: CL009BX

Cd8a mouse monoclonal antibody, clone 49-31.1, Biotin


USD 435.00

2 Weeks*

Size
    • 300 ug

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Specifications

Product Data
Clone Name 49-31.1
Applications Assay, FC
Recommended Dilution Flow Cytometry.
Reactivities Mouse
Host Mouse
Isotype IgG3
Clonality Monoclonal
Immunogen Immunization:
Recipient: 129/ReJ
Donor: CBA
Fusion Partner: Spleen from immunized recipient fused with Myeloma P3 NSI-Ag 4-1
Specificity Anti-mouse Ly-2.1 monoclonal antibody reacts with a sub-population of lymphocytes from mouse strains expressing the Ly 2.1 (CD8a) phenotype, but does not react with lymphocytes from mouse strains expressing the Ly 2.2 phenotype.
Formulation PBS containing 0.02% sodium azide (NaN3) as preservative and EIA grade BSA as a stabilizing protein to bring total protein concentration to 4-5 mg/ml.
Label: Biotin
State: Liquid purified Ig fraction
Concentration 0,1 mg/ml
Purification Affinity chromatography on Protein G
Conjugation Biotin
Gene Name Mus musculus CD8 antigen, alpha chain (Cd8a), transcript variant 1
Synonyms CD8 alpha chain, CD8A, MAL
Note Protocol: FLOW CYTOMETRY ANALYSIS:
Method:
1. Prepare a cell suspension in media A. For cell preparations, deplete the red blood cell population with Lympholyte®-M cell separation medium.
2. Wash 2 times.
3. Resuspend the cells to a concentration of 2x10e7 cells/ml in media A. Add 50 μl of this suspension to each tube (each tube will then contain 1 x 10e6 cells, representing 1 test).
4. To each tube, add 0.5-0.1 μg of this antibody per 10e6 cells.
5. Vortex the tubes to ensure thorough mixing of antibody and cells.
6. Incubate the tubes for 30 minutes at 4°C.
7. Wash 2 times at 4°C.
8. Add 100 μl of secondary antibody (Streptavidin-PE) at a 1/500 dilution.
9. Incubate tubes at 4°C for 30 - 60 minutes (It is recommended that tubes are protected from light since most fluorochromes are light sensitive).
10. Wash 2 times at 4°C.
11. Resuspend the cell pellet in 50 μl ice cold media B.
12. Transfer to suitable tubes for flow cytometric analysis containing 15 μl of propidium iodide at 0.5 mg/ml in PBS. This stains dead cells by intercalating in DNA.
Media:
A. Phosphate buffered saline (pH 7.2) + 5% normal serum of host species + sodium azide (100 μl of 2M sodium azide in 100 mls).
B. Phosphate buffered saline (pH 7.2) + 0.5% Bovine serum albumin + sodium azide (100 μl of 2M sodium azide in 100 mls).
Results:
Tissue Distribution by Flow Cytometry Analysis:
Mouse Strain: C3H/He
Cell Concentration: 1x10e6 cells per test
Antibody Concentration Used: 0.2 μg/10e6 cells
Percentage of cells stained above control:
Thymus 80.7%
Reference Data

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.