CD11a / ITGAL Mouse Monoclonal Antibody [Clone ID: 8-6.2]

CAT#: CL013R

CD11a / ITGAL mouse monoclonal antibody, clone 8-6.2, PE

Product Images

Cell Source: Lymph Node Percentage of cells stained above control: 99.6%

Specifications

Product Data

• Clone Name: 8-6.2
• Applications: FC
• Recommended Dilution: Flow Cytometry (See Protocols).
• Reactivities: Mouse
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: B6-Ly-1a Thymus, spleen and lymph node.
  Donor: 129/ReJ spleen
  Fusion Partner: P3-NS-1 Ag-4
• Specificity: This CD11a Monoclonal Antibody identifies a cell surface glycoprotein consisting of two non-covalently associated chains with molecular weights of 180kDa (Alpha chain) (1) present on most common lymphocytes and T and B cells.
• Formulation: PBS containing 0.02% Sodium Azide as preservative and EIA grade BSA as a stabilizing protein to bring total protein concentration to 4-5 mg/ml
  Label: PE
  State: Liquid purified Ig fraction
• Concentration: 0.1 mg/ml
• Purification: Protein G Chromatography
• Conjugation: PE
• Background: CD11a is a member of the integrin family of cell adhesion molecules. It is a glycoprotein expressed in combination with the CD18 beta chain. The complex is a member of the beta 2 integrin family. These molecules function in cell adhesion and specifically bind to CD54, ICAM2, ICAM3. CD11a is expressed on thymocytes, T and B lymphocytes, granulocytes, monocytes, and macrophages.
• Synonyms: Integrin alpha-L, LFA1, LFA-1
• Note: Protocol: Flow Cytometry Analysis:
  Method:
  1. Prepare a cell suspension in media A. For cell preparations, deplete the red blood cell population with Lympholyte®-M cell separation medium.
  2. Wash 2 times.
  3. Resuspend the cells to a concentration of 2x10e7 cells/ml in media A. Add 50 µl of this suspension to each tube (each tube will then contain 1x106 cells, representing
  1 test).
  4. To each tube, add ~2.0 µg* of CL013R or CL013RX.
  5. Vortex the tubes to ensure thorough mixing of antibody and cells.
  6. Incubate the tubes for 30 minutes at 4°C.
  (It is recommended that the tubes are protected from light since most fluorochromes are light sensitive).
  7. Wash 2 times at 4°C.
  8. Resuspend the cell pellet in 50 µl ice cold media B.
  9. Transfer to suitable tubes for flow cytometric analysis containing 15 µl of propidium iodide at 0.5 mg/ml in PBS. This stains dead cells by intercalating in DNA.
  
  Media:
  A. Phosphate buffered saline (pH 7.2) + 5% normal serum of host species + sodium azide (100 μl of 2M sodium azide in 100 mls).
  B. Phosphate buffered saline (pH 7.2) + 0.5% Bovine serum albumin + sodium azide (100 μl of 2M sodium azide in 100 mls).
  
  Results:
  Tissue Distribution by Flow Cytometry Analysis:
  Mouse Strain: CBA/J
  Cell Concentration : 1x10e6 cells per test
  Antibody Concentration Used: 2.0 µg/106 cells
  Isotypic Control: PE Mouse IgG2a
  
  Cell Source Percentage of cells stained above control:
  Thymus: 98.3%
  Lymph Node: 99.6
  
  Strain Distibution:
  Cell Concentration: 1x10e6 cells per test
  Antibody Concentration Used: 1.0 µg/10e6 cells
  Strains Tested: /6, BALB/c, AKR, CBA/J, C3H/HE
  Positive: C57BL/6, CBA/J, C3H/He
  Negative: BALB/c, AKR