CD45 / LCA (CD45RB) Rat Monoclonal Antibody [Clone ID: 16A]

CAT#: CL029RX

CD45 / LCA (CD45RB) rat monoclonal antibody, clone 16A, PE

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Specifications

Product Data

• Clone Name: 16A
• Applications: FC
• Recommended Dilution: Flow cytometry.
  Immunohistochemistry on Acetone-Fixed Frozen Sections
  Immunohistochemistry on Paraffin Embedded Sections.
  Immunoprecipitation.
  
  TEST RESULTS:
  Tissue Distribution by Flow Cytometry Analysis:
  Mouse Strain: BALB/c
  Cell Concentration: 1x10e6 cells per test
  Antibody Dilution Used: 0.5 μg/10e6 cells
  Percentage of cells stained above control:
  Thymus 96.8%
  Spleen 76.6%
  Lymph Node 66.5%
  Bone Marrow 29.2%
• Reactivities: Mouse
• Host: Rat
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: T_H2 cell clones, final boost with T_H2 clone D10.
  Fusion Partner: Ag8.653
• Specificity: Anti-Mouse CD45RB monoclonal antibody reacts with the CD45 isoform containing the exon B dependent epitope.
  CD45RB is highly expressed on peripheral B cells, cytotoxic T cells, a subset of T helper cells and most thymoctyes.
• Formulation: PBS containing EIA grade BSA as a stabilizing protein to bring total protein concentration to 4-5 mg/ml
  Label: PE
  State: Liquid purified IgG fraction
  Preservative: 0.02% Sodium Azide
  Label: R - Phycoerythrin
• Concentration: 0.1 mg/ml
• Purification: Protein G Chromatography
• Conjugation: PE
• Synonyms: PTPRC, Leukocyte common antigen, L-CA, T200