Thy1 Mouse Monoclonal Antibody [Clone ID: T11D7e]
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Specifications
Product Data | |
Clone Name | T11D7e |
Applications | CT, FC |
Recommended Dilution | Cytotoxic Assays. Flow Cytometry: Use 10 µl of 1/5 diluted antibody to label 106 cells in 100 µl. This product is routinely tested in Flow Cytometry using Mouse thymocytes. |
Reactivities | Mouse, Rat |
Host | Mouse |
Isotype | IgM |
Clonality | Monoclonal |
Immunogen | Xenogeneic rat thymocytes. Spleen cells from immunised CBA mice were fused with cells of the mouse NS1 myeloma cell line. |
Specificity | This antibody recognises Mouse and Rat CD90 also known as Thy1.1. Thy1.1 is a monomorphic determinant in rat but polymorphic in mice. Clone T11D7e reacts with Thy1.1 mice e.g. AKR and FVB mice, but not Thy1.2 mice such as CBA and BALB/c. Clone T11D7e is useful for removal of T lymphocytes from cell populations by complement mediated cytotoxicity. |
Formulation | PBS State: Purified State: Liquid purified IgM fraction Preservative: 0.09% Sodium Azide |
Concentration | 1.0 mg/ml |
Purification | Ammonium Sulphate Precipitation |
Database Link | |
Background | CD90 (Thy1) antigen is a GPI linked glycoprotein member of the Immunoglobulin superfamily. It is expressed on murine T cells, thymocytes, neural cells, cells of granulocytic lineage, early hematopoietic progenitors, fibroblasts, neurons and Kupffer's cells. Thy1 may play a role in cell to cell or cell to ligand interactions during synaptogenesis and other events in the brain. It is found in most mouse strains except AKR/J, A, Thy1.1 and B6.PL (74NS) expressing Thy1.1 |
Synonyms | Thy-1, THY1, CDw90 |
Reference Data |
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