Thy1 Mouse Monoclonal Antibody [Clone ID: 5a-8]

CAT#: CL039P

Thy1 mouse monoclonal antibody, clone 5a-8, Purified

Product Images

10 ug/ml staining CD19 in Human Skin by Immunohistochemistry, Formalin-Fixed Paraffin-Embedded tissue.

Specifications

Product Data

• Clone Name: 5a-8
• Applications: CT, FC, IHC
• Recommended Dilution: Immunohistochemistry on frozen sections.
  Cytotoxicity Analysis (see protocol).
• Reactivities: Mouse
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: CBA/J
• Specificity: This antibody reacts with all T lymphocytes from mouse strains expressing the Thy 1.2 phenotype (e.g. C57BL/6, C3H/He, DBA/2, CBA/J, BALB/c), but does not react with lymphocytes expressing the Thy 1.1 phenotype [e.g. AKR/J, B6.PL(74NS)].
• Formulation: PBS with 0.02% sodium azide as preservative.
  State: Purified
  State: Liquid IgG fraction.
  Label: APC conjugated.
• Concentration: 0.2 mg/ml
• Database Link:
  Entrez Gene 21838 Mouse
• Background: CD90 / Thy1 antigen is a GPI linked glycoprotein member of the Immunoglobulin superfamily. It is expressed on murine T cells, thymocytes, neural cells, cells of granulocytic lineage, early hematopoietic progenitors, fibroblasts, neurons and Kupffer's cells. Thy1 may play a role in cell to cell or cell to ligand interactions during synaptogenesis and other events in the brain. It is found in most mouse strains except AKR/J, A, Thy1.1 and B6.PL (74NS) expressing Thy1.1.
• Synonyms: Thy-1, THY1, CDw90
• Note: Protocol: CYTOTOXICITY ANALYSIS:
  
  Method:
  1. Prepare a cell suspension in media A. For cell preparations, deplete the red blood cell population with Lympholyte®-M cell separation medium.
  2. Wash 2 times.
  3. Resuspend the cells to a concentration of 2x10e7 cells/ml in media A. Add 50 µl of this suspension to each tube (each tube will then contain 1x10e6 cells,
  representing 1 test).
  4. To each tube, add 1.0 ug of this Ab per 10e6 cells.
  5. Vortex the tubes to ensure thorough mixing of antibody and cells.
  6. Incubate the tubes for 30 minutes at 4°C.
  (It is recommended that tubes are protected from light since most fluorochromes are light sensitive)
  7. Wash 2 times at 4°C.
  8. Resuspend the cell pellet in 50 µl ice cold media B.
  9. Transfer to suitable tubes for flow cytometric analysis containing 15 µl of propidium iodide at 0.5 mg/ml in PBS. This stains dead cells by intercalating in DNA.
  
  Media:
  A. Phosphate buffered saline (pH 7.2) + 5% normal serum of host species + sodium azide (100 µl of 2M sodium azide in 100 mls).
  B. Phosphate buffered saline (pH 7.2) + 0.5% Bovine serum albumin + sodium azide (100 µl of 2M sodium azide in 100 mls).
  
  Results-Tissue Distribution:
  Mouse Strain: BALB/c
  Cell Concentration : 1x10e6 cells per test
  Antibody Concentration Used: 1.0 ug / 10e6 cells
  Isotypic Control: APC Mouse IgG2b
  
  Cell Source-Percentage of cells stained above control:
  Thymus: 99.8%
  
  Strain Distribution:
  Tissue: Thymus
  Cell Concentration: 1x10e6 cells per test
  Antibody Concentration Used: 1.0 ug/10e6 cells
  Strains Tested: C57BL/6, C3H/He, CBA/J, BALB/c, ATL, AKR/J
  Positive: C57BL/6, C3H/He, CBA/J, BALB/c, ATL
  Negative: AKR