Ly6g Rat Monoclonal Antibody [Clone ID: RB6-8C5]

CAT#: CL046FX

Ly6g rat monoclonal antibody, clone RB6-8C5, FITC


USD 415.00

2 Weeks*

Size
    • 300 ug

Product Images

Specifications

Product Data
Clone Name RB6-8C5
Applications FC, WB
Recommended Dilution Flow cytometry (1,2,3).
Western blot (5).
Reactivities Mouse
Host Rat
Isotype IgG2b
Clonality Monoclonal
Specificity This antibody reacts with the Mouse myeloid differentiation antigen GR-1 (1,2).
Formulation PBS, 0.02% NaN3 and EIA grade BSA as a stabilizing protein to bring total protein concentration to 4-5 mg/ml
Label: FITC
State: Liquid Ig fraction
Absorption emission: 495 nm / 528 nm
Concentration 0.1 mg/ml
Purification Protein G chromatography
Conjugation FITC
Gene Name lymphocyte antigen 6 complex, locus G
Background GR-1 is a 25-30 kDa cell surface antigen and is expressed on myeloid cells but not lymphoid or erythroid cells. The expression of the Gr-1 antigen increases with granulocyte maturation (3) as shown by the distinct populations of bone-marrow cells this monoclonal antibody labels: negative, low positive and high positive. Expression is transient on cells of monocytic lineage (3).
Synonyms Gr-1 Granulocyte marker
Note Protocol: FLOW CYTOMETRY ANALYSIS:

Method:
1. Prepare a cell suspension in media A. For cell preparations, deplete the red blood cell population.
2. Wash 2 times.
3. Resuspend the cells to a concentration of 2x10e7 cells/ml in media A. Add 50 µl of this suspension to each tube (each tube will then contain 1 x 10e6 cells, representing 1 test).
4. To each tube, add 0.1 - 0.5 µg of antibody per 10e6 cells.
5. Vortex the tubes to ensure thorough mixing of antibody and cells.
6. Incubate the tubes for 30 minutes at 4°C. (It is recommended that the tubes are protected from light, since most fluorochromes are light sensitive.)
7. Wash 2 times at 4°C.
8. Resuspend the cell pellet in 50 µl ice cold media B.
9. Transfer to suitable tubes for flow cytometric analysis containing 15 µl of propidium iodide at 0.5 mg/ml in PBS. This stains dead cells by intercalating in DNA.

Media:
A. Phosphate buffered saline (pH 7.2) + 5% normal serum of host species + sodium azide (100 µl of 2M sodium azide in 100 mls).
B. Phosphate buffered saline (pH 7.2) + 0.5% Bovine serum albumin + sodium azide (100 µl of 2M sodium azide in 100 mls).

Results:
Tissue Distribution by Flow Cytometry Analysis:
Mouse Strain: CBA/J
Cell Concentration : 1x10e6 cells per test
Antibody Concentration Used: 0.1 µg/10e6 cells
Isotypic Control: FITC Rat IgG2b
Cell Source Percentage of cells stained above control:
Thymus 1.5%
Whole Blood Monocytes 87.2%
Bone Marrow Macrophages 90.0%

(see picture below)

Strain Distribution by Flow Cytometry Analysis:
Procedure: see above
Cell Concentration: 1x10e6 cells per test
Antibody Concentration Used: 0.1 µg/10e6 cells
Strains Tested: BALB/c, C57BL/6, CBA, C3H/he, AKR
Positive: BALB/c, C57BL/6, CBA, C3H/he, AKR
Negative: none
Reference Data

{0} Product Review(s)

0 Product Review(s) Submit review

Be the first one to submit a review

Product Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.