MHC Class II I-Abd Mouse Monoclonal Antibody [Clone ID: 28-16-8S]

CAT#: CL067R

MHC Class II I-Abd mouse monoclonal antibody, clone 28-16-8S, PE


USD 250.00

2 Weeks*

Size
    • 50 ug

Product Images

Specifications

Product Data
Clone Name 28-16-8S
Applications FC
Recommended Dilution Flow Cytometry (See Protocols).
Reactivities Mouse
Host Mouse
Isotype IgM
Clonality Monoclonal
Immunogen C3H.SW spleen
Donor: C3H
Fusion Partner: SP2/0Ag.14
Specificity Recognizes Mouse MHC class II I-Ab and cross reacts with I-Ad.
This Monoclonal Antibody reacts with the I-Ab encoded MHC class II antigen expressed on Mouse strains of the H-2b haplotype. It also reacts with the I-Ad encoded MHC class II antigen expressed on Mouse strains of the H-2d haplotype. Class II antigens are most highly expressed on antigen presenting cells including B cells, macrophages, dendritic cells and certain epithelial cells.
Formulation PBS containing 0.02% Sodium Azide as a preservative as a and EIA grade BSA as a stabilizing protein to bring total protein concentration to 4-5 mg/ml
Label: PE
State: Liquid purified IgM fraction
Concentration 0.1 mg/ml
Conjugation PE
Note Protocol: FLOW CYTOMETRY ANALYSIS:
Method:
1. Prepare a cell suspension in media A. For cell preparations, deplete the red blood cell population with Lympholyte®-M cell separation medium.
2. Wash 2 times.
3. Resuspend the cells to a concentration of 2x10e7 cells/ml in media A. Add 50 µl of this suspension to each tube (each tube will then contain 1x10e6 cells, representing 1 test).
4. To each tube, add~0.2 µg of CL067R per 10e6 cells.
5. Vortex the tubes to ensure thorough mixing of antibody and cells.
6. Incubate the tubes for 30 minutes at 4°C.
(It is recommended that the tubes are protected from light, since most fluorochromes are light sensitive).
7. Wash 2 times at 4°C.
8. Resuspend the cell pellet in 50 µl ice cold media B.
9. Transfer to suitable tubes for flow cytometric analysis containing 15 µl of propidium iodide at 0.5 mg/ml in PBS. This stains dead cells by intercalating in DNA.

Media:
A. Phosphate buffered saline (pH 7.2) + 5% normal serum of host species + sodium azide (100 ml of 2M sodium azide in 100 mls).
B. Phosphate buffered saline (pH 7.2) + 0.5% Bovine serum albumin + sodium azide (100 ml of 2M sodium azide in 100 mls).

Results:
Tissue Distribution by Flow Cytometry Analysis:
Mouse strain: C57BL/6
Cell Concentration: 1x10e6 cells per test.
Antibody Concentration Used: 0.2 µg/10e6 cells.
Isotypic Control: PE Mouse IgM

Cell Source: Percentage Stained Above Control:
Thymus: 28.2%
Spleen: 40.7%
Reference Data

{0} Product Review(s)

0 Product Review(s) Submit review

Be the first one to submit a review

Product Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.