CD44 Mouse Monoclonal Antibody [Clone ID: OX-49]

CAT#: CL110F

CD44 mouse monoclonal antibody, clone OX-49, FITC


USD 270.00

2 Weeks*

Size
    • 100 ug

Product Images

Specifications

Product Data
Clone Name OX-49
Applications FC, IHC
Recommended Dilution Flow cytometry (See protocol). 
Immunohistochemistry on Frozen Sections and Paraffin Sections.
Reactivities Rat
Host Mouse
Isotype IgG2a
Clonality Monoclonal
Immunogen T cell blasts.
Specificity This anti-Rat CD44 monoclonal antibody recognizes an epitope on both standard CD44 and its splice variant.
Formulation PBS with 0.02% sodium azide as preservative and EIA grade BSA as a stabilizer
Label: FITC
State: Liquid purified IgG fraction
Concentration 0.1 mg/ml
Purification Protein G affinity chromatography
Conjugation FITC
Background This antigen is expressed on most leukocytes (except a sub population of B cells) and increases upon activation. This antibody binds extracellularly to the standard (S) form on rat leukocytes, but it is not known if they bind to the N-terminal region. It has also been reported that the antibody may bind to melanoma cell lines that express CD44V (splice variant form).
CD44 is expressed on most leukocytes except a sub population of B cells. Its expression is increased on T and B blasts.
Synonyms LHR, MDU2, MDU3, MIC4, CDw44, Epican, ECMR-III, HUTCH-I, Heparan sulfate proteoglycan, Hermes antigen, Hyaluronate receptor, PGP-1
Note Protocol: FLOW CYTOMETRY ANALYSIS:

Method:
1. Prepare a cell suspension in media A. For cell preparations, deplete the red blood cell population with Rat cell separation medium.
2. Wash 2 times.
3. Resuspend the cells to a concentration of 2x107 cells/ml in media A. Add 50 µl of this suspension to each tube (each tube will then contain 1x106 cells, representing 1 test).
4. To each tube, add 1.0-0.5 µg* of CL110F or CL110FX.
5. Vortex the tubes to ensure thorough mixing of antibody and cells.
6. Incubate the tubes for 30 minutes at 4°C.
(It is recommended that the tubes are protected from light since most fluorochromes are light sensitive).
7. Wash 2 times at 4°C.
8. Resuspend the cell pellet in 50 µl ice cold media B.
9. Transfer to suitable tubes for flow cytometric analysis containing 15 µl of propidium iodide at 0.5 mg/ml in PBS. This stains dead cells by intercalating in DNA.

Media:
A. Phosphate buffered saline (pH 7.2) + 5% normal serum of host species + sodium azide (100 µl of 2M sodium azide in 100 mls).
B. Phosphate buffered saline (pH 7.2) + 0.5% Bovine serum albumin + sodium azide (100 µl of 2M sodium azide in 100 mls).

Results-Tissue Distribution:
Rat Strain: Wistar
Cell Concentration: 1 x 10e6 cells per tests
Antibody Concentration Used: 1.0 µg/10e6 cells
Isotypic Control: FITC Mouse IgG2a.

Cell Source Percentage of cells stained above control:
Thymus: 99.6%
Spleen: 75.9%
Lymph Node: 96.8%

N.B. Appropriate control samples should always be included in any labelling studies.
* For optimal results in various applications, it is recommended that each investigator determine dilutions appropriate for individual use.
Reference Data

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.