MHC Class I (RT1Ac) Mouse Monoclonal Antibody [Clone ID: OX-27]

CAT#: CL129R

MHC Class I (RT1Ac) mouse monoclonal antibody, clone OX-27, PE

Specifications

Product Data

• Clone Name: OX-27
• Applications: FC
• Recommended Dilution: Flow cytometry: use 10 µl of neat antibody to label 10e6 cells in 100 µl.
• Reactivities: Rat
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: PHA activated rat lymphocytes
• Specificity: This antibody recognises a polymorphic determinant (c haplotype) of rat Class I MHC Antigen (RT-1A).
• Formulation: PBS containing 1% BSA and 0.09% sodium azide
  Label: PE
  State: Lyophilized purified IgG fraction
  Label: R. Phycoerythrin (RPE)
• Reconstitution Method: Restore with 1 ml distilled water.
• Purification: Protein G chromatography
• Conjugation: PE
• Background: MHC Class I molecules play a central role in the immune system by presenting peptides derived from the endoplasmic reticulum lumen. MHC class I antigens are heterodimers consisting of one alpha chain (44kDa) with beta 2 microglobulin (11.5 kDa). The antigen is expressed by all somatic cells at varying levels. MHC Class I molecules are expressed on most nucleated cells where they present endogenously synthesized antigenic peptides to CD8+ T lymphocytes, which are usually cytotoxic T cells. Fibroblasts or neurons however only show a low level of antigen.
• Synonyms: RT1-A3