C3 Rat Monoclonal Antibody [Clone ID: RMC11H9]

CAT#: CL200P

C3 rat monoclonal antibody, clone RMC11H9, Purified

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Specifications

Product Data

• Clone Name: RMC11H9
• Applications: ELISA, FC, IF, IHC, WB
• Recommended Dilution: ELISA.
  Western blot.
  Flow Cytometry.
  Immunofluorescence.
  Immunohistochemistry on Acetone Fixed Frozen Sections.
• Reactivities: Mouse
• Host: Rat
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: C57BL/6 thymocytes incubated with a rat IgG2b anti-murine Thy-1 (RmT1) and C57BL/6 serum (as a source for C1q).
  Donor: Lou/c rat spleen cells.
  Fusion Partner: Mouse myeloma cell line P3X63 Ag. 8.653.
• Specificity: This antibody recognizes C3 as well as the breakdown products C3b, iC3b and C3dg.
  
  Results of Tissue Distribution by Flow Cytometry Analysis:
  Mouse strain: C57Bl/6
  Cell concentration : 1x10⁶ cells per test
  Antibody Concentration Used: 1.0 µg/ 10⁶ cells
  Secondary Antibody: Goat anti-Rat IgG FITC (1/500 dilution)
  Cell Source: Thymocytes incubated with anti-Mouse CD90 (Cat.-No CL039P), then incubated with fresh mouse serum (same strain) and stained as indicated below
  Percentage of cells stained above control: 67.4% (see picture below)
• Formulation: PBS containing 0.02% Sodium Azide as preservative.
  State: Purified
  State: Liquid purified IgG fraction.
• Concentration: 1.0 mg/ml
• Purification: Protein G Chromatography.
• Gene Name: complement component 3
• Database Link:
  Entrez Gene 12266 Mouse
• Background: C3 is the most abundant complement protein in serum. C3 and its cleavage products, C3a and C3b, play a central role in the complement activation cascade. C3b forms an integral part of the C3 and C5 convertases as it promotes complement activation and the subsequent formation of the membrane attack complex. C3a possesses anaphlatoxic as well as various immunoregulatory properties. Also, C3 has been implicated in developmental and non-imflammatory process such as hematopoiesis, skeletal and vascular development and reproduction.
• Synonyms: CPAMD1, Complement component 3