CLEC10A Mouse Monoclonal Antibody [Clone ID: 125A10.03]

CAT#: DDX0010A546-100

CLEC10A mouse monoclonal antibody, clone 125A10.03

Product Images

FACS analysisof monocyte-derivedDCs (GM+IL4) stainedwith 125A10.03+ goat anti-mouse FITC

Specifications

Product Data

• Clone Name: 125A10.03
• Applications: IF
• Recommended Dilution: IF
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: human ASGPR Ig fusion protein
• Specificity: human ASGPR/MGL
• Formulation: Label: Alexa Fluor 546
  State: 100 μg in 200 μl / 50 μg in 100 μl PBS 50% glycerol
• Concentration: lot specific
• Conjugation: Alexa Fluor 546
• Storage: -20°C. KEEP CONTENTS STERILE: no preservative.
  Coupled antibodies: glycerol protects from freezing.
• Gene Name: C-type lectin domain family 10 member A
• Database Link:
  Entrez Gene 10462 Human
  UniProt ID Q8IUN9
• Background: ASGPR/MGL (asialoglycoprotein receptor/ macrophage galactose lectin receptor) / CD301 is a Ca²⁺ dependent type II transmembrane lectin. In search for genes specifically expressed by dendritic cells (DC), we have cloned several cDNAs encoding different forms of asialoglycoprotein receptor (ASGPR), that were expressed in eukaryotic cells for use as immunogens. Immunoprecipitation from DCs using anti-DCASGPR mAb yields a major 40-kDa protein. mAb 125A10.03 recognized the ASGPR isoform expressed by DC but not the isoform expressed by monocytes. This antibody stained CD14⁺derived DCs obtained from CD34⁺ progenitors, but not the CD1a⁺derived subset. Accordingly, both monocyte-derived DCs and tonsillar interstitial-type DCs express DC-ASGPR protein, while Langerhans-type cells do not. DC-ASGPR is a feature of immaturity, as its expression is lost upon CD40L activation. In agreement with the presence of tyrosine-based and dileucine motifs in the intracytoplasmic domain, mAb against DC-ASGPR is rapidly internalized by DCs at 37°C. Finally, intracellular DC-ASGPR is localized in early endosomes, suggesting that the receptor recycles to the cell surface following internalization of ligand. (Valladeau J et al, 2001, J. Immunol., 167: 5767).
• Synonyms: CLECSF13, CLECSF14, HML