CLEC10A Mouse Monoclonal Antibody [Clone ID: 121A5.01]

CAT#: DDX0011A546-100

CLEC10A mouse monoclonal antibody, clone 121A5.01


USD 530.00

2 Weeks*

Size
    • 100 ug

Product Images

Specifications

Product Data
Clone Name 121A5.01
Applications IF
Recommended Dilution IF
Reactivities Human
Host Mouse
Isotype IgG1
Clonality Monoclonal
Immunogen human ASGPR Ig fusion protein
Specificity human ASGPR/MGL
Formulation Label: Alexa Fluor 546
State: 100 μg in 200 μl / 50μg in 100 μl PBS 50% glycerol
Concentration lot specific
Purification QMA Hyper D ion exchange chromatography
Conjugation Alexa Fluor 546
Storage -20°C. KEEP CONTENTS STERILE: no preservative.
Coupled antibodies: glycerol protects from freezing.
Gene Name C-type lectin domain family 10 member A
Background ASGPR/MGL (asialoglycoprotein receptor / macrophage galactose lectin receptor) / CD301 is a Ca2+ dependent type II transmembrane lectin. In search for genes specifically expressed by dendritic cells (DC), we have cloned several cDNAs encoding different forms of asialoglycoprotein receptor (ASGPR), that were expressed in eukaryotic cells for use as immunogens. Immunoprecipitation from DCs using anti-DCASGPR mAb yields a major 40-kDa protein. mAb 121A5.01 recognized the ASGPR isoform expressed by DC but not the isoform expressed by monocytes. This antibody stained CD14+derived DCs obtained from CD34+ progenitors, but not the CD1a+derived subset. Accordingly, both monocyte-derived DCs and tonsillar interstitial-type DCs express DC-ASGPR protein, while Langerhans-type cells do not. DC-ASGPR is a feature of immaturity, as its expression is lost upon CD40L activation. In agreement with the presence of tyrosine-based and dileucine motifs in the intracytoplasmic domain, mAb against DC-ASGPR is rapidly internalized by DCs at 37°C. Finally, intracellular DC-ASGPR is localized in early endosomes, suggesting that the receptor recycles to the cell surface following internalization of ligand. (Valladeau J et al, 2001, J. Immunol., 167: 5767).
Synonyms CLECSF13, CLECSF14, HML
Reference Data

{0} Product Review(s)

0 Product Review(s) Submit review

Be the first one to submit a review

Product Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.