Ly75 Rat Monoclonal Antibody [Clone ID: NLDC-145]

CAT#: DDX0020A488-100

Ly75 rat monoclonal antibody, clone NLDC-145

Specifications

Product Data

• Clone Name: NLDC-145
• Applications: FC, IF
• Recommended Dilution: Flow Cytometry, IF
• Reactivities: Mouse
• Host: Rat
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Mouse lymphoid tissue stroma
• Specificity: Mouse dendritic cells
• Formulation: PBS 50% glycerol
  Label: Alexa Fluor 488
• Concentration: lot specific
• Purification: QMA Hyper D ion exchange chromatography
• Conjugation: Alexa Fluor 488
• Storage: -20°C. KEEP CONTENTS STERILE: no preservative.
  Purified antibodies: avoid repeated freeze/thaw cycles.
  Coupled antibodies: glycerol protects from freezing.
• Gene Name: lymphocyte antigen 75
• Database Link:
  Entrez Gene 17076 Mouse
  UniProt ID Q60767
• Background: NLDC-145 has been described as reacting specifically with a group of nonlymphoid dendritic cells including Langerhans cells (LC), veiled cells (VC), and interdigitating cells (IDC). The antibody does not react with precursor cells in bone marrow and blood. Macrophages are not stained by the antibody, but a subpopulation of Ia+ peritoneal exudate cells is recognized. It stains DC in T lymphocyte areas, including spleen white pulp but not spleen marginal zone DC or LC. The Ag is also expressed on activated macrophages and thymic cortical epithelium. DEC-205 has some similarities with mannose receptors and acts as a receptor for Ag uptake. (Kraal G., et al, J.Exp.med. 1986, 163, 981 ; Steinman RM, et al, PNAS, 2002, 99, 351-358 ; Flores-Langarica et al, PNAS, 2005, 102, 19039-19044) ATCC ref: HB-290TM.
• Synonyms: LY75, CLEC13B, DEC205, gp200-MR6