CD14 Mouse Monoclonal Antibody [Clone ID: MOP9.25]

CAT#: DDX0100P-100

CD14 mouse monoclonal antibody, clone MOP9.25

Product Images

Human PBMC stained with MOP9.25

Specifications

Product Data

• Clone Name: MOP9.25
• Applications: FC, IHC
• Recommended Dilution: Flow cytometry, cell depletion, IHC.
  Usage recommendation:
  *This monoclonal antibody may be used between 1-5 μg/ml for flow cytometry and 5-25 μg/ml for IHC
  
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: peripheral blood monocytes (patient with rheumatoid arthritis)
• Specificity: human CD14
• Formulation: State: Purified
  State: 100 μg in 200 μl / 50 μg in 100 μl Tris-NaCl pH 8
• Concentration: lot specific
• Purification: QMA Hyper D ion exchange chromatography
• Conjugation: Unconjugated
• Storage: -20°C. KEEP CONTENTS STERILE: no preservative.
  Purified antibodies: avoid repeated freeze/thaw cycles.
• Gene Name: CD14 molecule
• Database Link:
  Entrez Gene 929 Human
  UniProt ID P08571
• Background: CD14 recognizes a human monocyte/macrophage antigen of 55 kDa. It reacts with blood monocytes and has a weak reactivity on blood granulocytes, and interfollicular macrophages. Different cytokines have an effect on CD14 expression: CD14 expression was studied in dendritic cells generation in vitro. After 5 days in GMCSF + IL4, CD14 disappears while CD1a expression appears. TNF-α skews monocyte differentiation form macrophages to dendritic cells. The antibody MOP9.25 is used for monocyte depletion before dendritic cells sorting. (Dimitriu-Bona A., et al, J. Immunol., 1983 ; 130: 145-152 ; Banchereau J. et al, Annu. Rev. Immunol., 2000; 18: 767-811; Chomarat P. et al, J. Immunol., 2003; 171: 2262-2269).
• Synonyms: CD14