CD40 Mouse Monoclonal Antibody [Clone ID: MAB89]

CAT#: DDX0131A488-100

CD40 mouse monoclonal antibody, clone MAB89

Product Images

CD40-transfected L cells stained with mAb89 (DDX0131)

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  IHC staining of human lymph node frozen section with clone mAb89 (DDX0131)

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  IHC staining of human tonsil frozen section with clone mAb89 (DDX0131)

Specifications

Product Data

• Clone Name: MAB89
• Applications: FC, IF
• Recommended Dilution: FACS surface, ImmunoFluorescence
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: anti-μ activated tonsillar human B cells
• Specificity: human CD40
• Formulation: 100 μg in 200 μl / 50 μg in 100 μl PBS 50% glycerol
  Label: Alexa Fluor 488
• Concentration: lot specific
• Purification: QMA Hyper D ion exchange chromatography
• Conjugation: Alexa Fluor 488
• Storage: -20°C. KEEP CONTENTS STERILE: no preservative.
  Purified antibodies: avoid repeated freeze/thaw cycles.
  Coupled antibodies: glycerol protects from freezing.
• Gene Name: CD40 molecule
• Database Link:
  Entrez Gene 958 Human
  UniProt ID P25942
• Background: CD40 (48 to 50 kDa) is a transmembrane glycoprotein mainly expressed on the surface of B cells and also expressed on monocytes, dendritic cells, and thymic epithelium. CD40 is a member of the tumor necrosis factor (TNF) receptor superfamily, which includes the low affinity nerve growth factor (NGF) receptor and CD95/Fas. CD40 is the receptor for CD40 ligand. CD40L (CD40L, CD154, gp39, and TRAM) belongs to the TNF gene family and is expressed more widely than CD40, predominantly on activated CD4+ T cells. Following interaction with CD40 ligand, CD40 mediates a number of major immunoregulatory functions, central to the control of thymus dependent humoral immunity and may be critical in the development of cell mediated immune responses. Other biological actions include B cell homotypic adhesion, proliferation, immunoglobulin isotype switch, and secretion. Activation of CD40 has also been shown to inhibit the growth of certain B cell lymphomas and to induce the death of transformed cells of mesenchymal or epithelial origin.
  (Vallé A. et al, 1989; Eur. J. Immunol., 19: 1463-1469; O’ Sullivan B. et al, 2003; Crit. Rev. Immunol., 23: 83-107)
• Synonyms: TNFRSF5, CDw40, Bp50