CD40L (CD40LG) Mouse Monoclonal Antibody [Clone ID: LL2]

CAT#: DDX0141A647-100

CD40L (CD40LG) mouse monoclonal antibody, clone LL2

Product Images

IF staining of hCD40L-transfected cells with LL2 clone

• 

  FACS staining of hCD40L-transfected L cells with LL2 clone

Specifications

Product Data

• Clone Name: LL2
• Applications: FC, IF
• Recommended Dilution: FACS surface, ImmunoFluorescence.
  *This monoclonal antibody may be used between 5-20 μg/ml.
  
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Chimeric Human CD40L-Ig-transfected COS-7 cells.
• Specificity: human CD40L.
• Formulation: 100 μg in 200 μl / 50 μg in 100 μl PBS 50% glycerol
  Label: Alexa Fluor 647
• Concentration: lot specific
• Purification: QMA Hyper D ion exchange chromatography
• Conjugation: Alexa Fluor 647
• Storage: -20°C. KEEP CONTENTS STERILE: no preservative.
  Purified antibodies: avoid repeated freeze/thaw cycles.
  Coupled antibodies: glycerol protects from freezing.
• Gene Name: CD40 ligand
• Database Link:
  Entrez Gene 959 Human
  UniProt ID P29965
• Background: CD40 ligand also known as TRAP, gp39, TNFSF5, is a 261 amino acid type II transmembrane glycoprotein belonging to the TNF superfamily. CD40 ligand is expressed predominantly on activated CD4+ T cells and is also found on other cell types, including NK cells, mast cells, basophils and eosinophils. Genetic alterations in the CD40L results in the absence of a functional molecule on activated T cells and are responsible for the occurrence of X-linked hyper-IgM syndrome. LL2 clone was produced by immunizing mice with L cells transfected with human CD40L. Addition of anti-CD40L mAb LL2 enhanced proliferation of MT9 and FS35 T cell lines in the presence of IL-2 and increased the secretion of IFN-γ by MT9 cells and of IL-10, IL-5 and IL-4 by FS35 cells cultured with IL-2. LL2 completely blocks CD40- CD40L interaction, can mimic T cell-induced CD40 triggering on B cells and activates T cells.
  (Hermann P et al, 1995, EJI, 25 (10), 2972-2977; Razanajaona D et al, 1996, J Immunol., 157(4):1492-8 ; Fournier N et al, 2000 J Immunol., 165: 1197–1209; Henn et al, 2001, Blood, 98 (4), 1047-1054).
• Synonyms: CD40 ligand, CD40-L, CD40LG, TNFSF5, TRAP, GP39