ICAM1 Mouse Monoclonal Antibody [Clone ID: 217E2.10]

CAT#: DDX0151A546-100

ICAM1 mouse monoclonal antibody, clone 217E2.10

Product Images

IHC staining of human lung frozen section with clone 217E2 (DX0151)

Specifications

Product Data

• Clone Name: 217E2.10
• Applications: FC, IF
• Recommended Dilution: DDX0151P-50 / DDX0151P-100 Purified: Flow cytometry, IHC (cryosection, paraffin), IF.
  DDX0151A488-50 / DDX0151A488-100 Alexa-fluor® 488: Flow cytometry, IF ( On request).
  DDX0151A546-50 / DDX0151A546-100 Alexa- fluor® 546: Flow cytometry, IF ( On request).
  DDX0151A647-50 / DDX0151A647-100 Alexa- fluor® 647: Flow cytometry, IF ( On request).
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: in vitro-derived DCs (GM-CSF+TNF )
• Formulation:
  Purified: 100 μg in 200 μl / 50 μg in 100 μl Tris-NaCl pH 8.
  Coupled: 100 μg in 200 μl / 50 μg in 100 μl PBS 50% glycerol
  Label: Alexa Fluor 546
• Concentration: lot specific
• Conjugation: Alexa Fluor 546
• Storage: -20°C. KEEP CONTENTS STERILE: no preservative.
  Purified antibodies: avoid repeated freeze/thaw cycles.
  Coupled antibodies: glycerol protects from freezing.
• Gene Name: intercellular adhesion molecule 1
• Database Link:
  Entrez Gene 3383 Human
  UniProt ID P05362
• Background: 217E2 monoclonal antibody was obtained following mouse immunization with in vitro-derived human dendritic cells. The target antigen was found to be CD54/ICAM-1, a cell surface glycoprotein which is typically expressed on endothelial cells and immune cells. CD54/ICAM-1, member of the Ig superfamily, binds to integrins of type CD11a / CD18, or CD11b / CD18 and is also a receptor for rhinovirus. CD54/ICAM-1 is a type of intracellular adhesion molecule, playing a role in transmigration of endothelial cells into tissues through binding to LFA-1.
  (Carlson M.et al, 1988, Nucleic Acids Res. 16 (9): 4188; Yang L. et al, 2005, Blood 106 (2): 584).
• Synonyms: ICAM-1