Lamp3 Rat Monoclonal Antibody [Clone ID: 1006F7.05]

CAT#: DDX0192A546-100

Lamp3 rat monoclonal antibody, clone 1006F7.05

Product Images

Immunostaining of murineintestine cryosections fixed with Acetone or PFA. IL: intestinal lumen; LT: lymphoid tissue

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  IHC staining of mouse lung frozen section with clone 1006F7 (DX0192)

Specifications

Product Data

• Clone Name: 1006F7.05
• Applications: FC, IF
• Recommended Dilution: DDX0192P-50 / DDX0192P-100 Purified: Surface and Intra Flow cytometry, IHC, IF.
  DDX0192A488-50 / DDX0192A488-100 Alexa-fluor®488: Surface and Intra Flow cytometry, IF.
  DDX0192A546-50 / DDX0192A546-100 Alexa- fluor®546: Surface and Intra Flow cytometry.
  DDX0192A647-50 / DDX0192A647-100 Alexa- fluor®647: Surface and Intra Flow cytometry.
  DDX0192B-50 / DDX0192B-100: Biotin (on request): Surface and Intra Flow cytometry, IHC.
  Usage recommendation:
  *This monoclonal antibody may be used between 5-20μg/ml.
  *Optimal dilution should be determined by each laboratory for each application.
  *Coupled antibody: to maintain RT before use.
• Reactivities: Mouse
• Host: Rat
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: recombinant murine DC-LAMP
• Specificity: Murine DC-LAMP
  Species cross-reactivity: none.
• Formulation: Purified: 100 μg in 200μl / 50 μg in 100 μl Tris-NaCl pH 8.
  Coupled: 100 μg in 200μl / 50 μg in 100 μl PBS 50% glycerol
  Label: Alexa Fluor 546
• Concentration: lot specific
• Purification: QMA hyper D ion exchange chromatography
• Conjugation: Alexa Fluor 546
• Storage: -20°C. KEEP CONTENTS STERILE: no preservative.
  Purified antibodies: avoid repeated freeze/thaw cycles.
  Coupled antibodies: glycerol protects from freezing.
• Gene Name: lysosomal-associated membrane protein 3
• Database Link:
  Entrez Gene 239739 Mouse
  UniProt ID Q7TST5
• Background: The dendritic cell lysosomal-associated membrane protein (DC-LAMP)/CD208 is a type I integral transmembrane glycoprotein mostly homologous to CD68, of about 45 kDa in the mouse and 90 kDa in human (due to extensive glycosylation), with a bipartite C-terminal structure divided by a serine/proline rich region, a transmembrane domain and a conserved tyrosine-based lysosomal targeting motif in its cytoplasmic tail. DC-LAMP is specifically expressed by human DCs upon activation. However, its mouse counterpart has not be detected in mature DCs. DC-LAMP is constitutively expressed by mouse. Confocal and immunoelectron microscopy have shown that mouse DC-LAMP protein co-localizes with lbm180, a specific marker for the limiting membrane of lamellar bodies that contains lung surfactant protein B.
  (Salaun B et al, 2003 ; Eur. J. Immunol., 33 :9, 2619-29 ; Salaun B et al, 2004 ; Am. J. Pathol., 164 :3, 861-71 ; Rochereau N et al, Vaccine, 2011).
• Synonyms: DCLAMP, DC-LAMP, TSC403, Dendritic Cell Marker