LAMP3 Mouse Monoclonal Antibody [Clone ID: 109G3]

CAT#: DDX0193A546-100

LAMP3 mouse monoclonal antibody, clone 109G3

Product Images

Cryosection of human lung type II pneumocytes stained with purified 109G3 +goat anti-mouse-HRP

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  IHC staining of human lung frozen section with clone 109G3 (DX0193)

Specifications

Product Data

• Clone Name: 109G3
• Applications: IF
• Recommended Dilution: DDX0193P-50 / DDX0193P-100 Purified: FACS intracellular, ImmunoHistoChemistry frozen sections.
  DDX0193A488-50 / DDX0193A488-100 Alexa-fluor®488: FACS intracellular, Immunofluorescence.
  DDX0193A546-50 / DDX0193A546-100 Alexa-fluor®546: Immunofluorescence.
  DDX0193A647-50 / DDX0193A647-100 Alexa-fluor®647: FACS intracellular.
  DDX0193B-50 / DDX0193B-100 Biotin (on request): FACS intracellular, ImmunoHistoChemistry frozen sections.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human DC-LAMP alkaline phosphatase fusion protein.
• Specificity: Human DC-LAMP (epitope in intracytoplasmic domain).
• Formulation: Purified: 100 μg in 200μl / 50 μg in 100 μl Tris-NaCl pH 8.
  Coupled: 100 μg in 200μl / 50 μg in 100 μl PBS 50% glycerol
  Label: Alexa Fluor 546
• Concentration: lot specific
• Purification: QMA Hyper D ion exchange chromatography
• Conjugation: Alexa Fluor 546
• Storage: -20°C. KEEP CONTENTS STERILE: no preservative.
  Purified antibodies: avoid repeated freeze/thaw cycles.
  Coupled antibodies: glycerol protects from freezing.
• Gene Name: lysosomal associated membrane protein 3
• Database Link:
  Entrez Gene 27074 Human
  UniProt ID Q9UQV4
• Background: The dendritic cell lysosomal-associated membrane protein (DC-LAMP)/CD208 is a type I integral transmembrane glycoprotein mostly homologous to CD68, of about 45 kDa in the mouse and 90 kDa in human (due to extensive glycosylation), with a bipartite C-terminal structure divided by a serine/proline rich region, a transmembrane domain and a conserved tyrosine-based lysosomal targeting motif in its cytoplasmic tail. Initially cloned as a specific marker of human mature dendritic cells (DCs), DC-LAMP has been subsequently shown to be expressed in alveolar type II pneumocytes. In both cell types, the molecule is found in the limiting membrane of intracellular multi-lamellar bodies, known as MIIC (MHC class II compartments) in human mature DC and as lung surfactant-containing lamellar bodies in type II pneumocytes. In the latter cell type, DC-LAMP expression is also detected at the cell surface.
  (de Saint-Vis B et al, 1998 ; Immunity ; 9: 325-336)
• Synonyms: DCLAMP, DC-LAMP, TSC403, Dendritic Cell Marker