LAMP3 Mouse Monoclonal Antibody [Clone ID: 208B5]

CAT#: DDX0195A488-100

LAMP3 mouse monoclonal antibody, clone 208B5

Product Images

Intracellular flow cytometry with anti-DC-LAMP at 5μg/ml on human monocyte-derived DC GM+ IL4 (24h in LPS)

Specifications

Product Data

• Clone Name: 208B5
• Applications: FC
• Recommended Dilution: DDX0195P-50 / DDX0195P-100 Purified: FACS intracellular, ImmunoHistoChemistry frozen sections.
  DDX0195A488-50 / DDX0195A488-100 Alexa-fluor®488: Intra Flow cytometry for mouse and human.
  DDX0195A546-50 / DDX0195A546-100 Alexa-fluor®546: FACS intracellular, ImmunoHistoChemistry frozen sections.
  DDX0195A647-50 / DDX0195A647-100 Alexa-fluor®647: Intra Flow cytometry for mouse and human.
  DDX0195B-50 / DDX0195B-100 Biotin: FACS intracellular, ImmunoHistoChemistry frozen sections.
  
  Usage recommendation: *This monoclonal antibody may be used between 5-10 μg/ml.
• Reactivities: Human, Mouse
• Host: Mouse
• Isotype: IgG3
• Clonality: Monoclonal
• Immunogen: Recombinant Murine DC-LAMP
• Specificity: Murine DC-LAMP (epitope in intracytoplasmic domain).
  Species cross-reactivity: Human.
• Formulation: Purified: 100 μg in 200 μl / 50 μg in 100 μl Tris-NaCl pH 8.
  Coupled: 100 μg in 200 μl / 50 μg in 100 μl PBS 50% glycerol.
  Label: Alexa Fluor 488
• Concentration: lot specific
• Purification: QMA Hyper D Ion exchange chromatography
• Conjugation: Alexa Fluor 488
• Storage: -20°C. KEEP CONTENTS STERILE: no preservative.
  Purified antibodies: avoid repeated freeze/thaw cycles.
  Coupled antibodies: glycerol protects from freezing.
• Gene Name: lysosomal associated membrane protein 3
• Database Link:
  Entrez Gene 27074 Human
  UniProt ID Q9UQV4
• Background: The dendritic cell lysosomal-associated membrane protein (DC-LAMP)/CD208 is a type I integral transmembrane glycoprotein mostly homologous to CD68, of about 45 kDa in the mouse and 90 kDa in human (due to extensive glycosylation), with a bipartite C-terminal structure divided by a serine/proline rich region, a transmembrane domain and a conserved tyrosine-based lysosomal targeting motif in its cytoplasmic tail. DC-LAMP is specifically expressed by human DCs upon activation. However, its mouse counterpart has not be detected in mature DCs. DC-LAMP is constitutively expressed by mouse. Confocal and immunoelectron microscopy has shown that mouse DC-LAMP protein co-localizes with lbm180, a specific marker for the limiting membrane of lamellar bodies that contains lung surfactant protein B (Salaun B et al, 2004; Eur. J. Immunol., 33: 2619-29).
• Synonyms: DCLAMP, DC-LAMP, TSC403, Dendritic Cell Marker