DC SIGN (CD209) (Extracell. Dom.) Mouse Monoclonal Antibody [Clone ID: 118A8.05]

CAT#: DDX0200A647-100

DC SIGN (CD209) (Extracell. Dom.) mouse monoclonal antibody, clone 118A8.05

Product Images

IHC staining of human skin frozen section with clone 118A8 (DX0200)

• 

  DC-SIGN-like expression on monocyte-derived DCs subsets. Blood monocytes were cultured in different conditions to obtain CD1a+ cells or CD14+ cells. Subsets were analyzed forDC-SIGN-like expression. (MuellerC., Courtesy)

Specifications

Product Data

• Clone Name: 118A8.05
• Applications: FC
• Recommended Dilution: Surface Flow cytometry
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: human (GMCSF + TNF) DC subset
• Specificity: human (epitope in extracellular domain
• Formulation: Label: Alexa Fluor 647
  State: 100 μg in 200 μl / 50 μg in 100 μl PBS 50% glycerol
• Concentration: lot specific
• Conjugation: Alexa Fluor 647
• Storage: -20°C. KEEP CONTENTS STERILE: no preservative.
  Coupled antibodies: glycerol protects from freezing.
• Gene Name: CD209 molecule
• Database Link:
  Entrez Gene 30835 Human
  UniProt ID Q9NNX6
• Background: We have generated an antibody recognizing an antigen which has a tissular distribution and a size (western blot) identical to DC-SIGN. This antibody does not recognize DC-SIGN 1 expressed in HeLa cells. DCSIGN (“DC Specific, ICAM-3 Grabbing, Nonintegrin”) / CD209 is a type II membrane protein with an external mannose-binding C-type lectin domain, DC-SIGN is expressed by immature and mature dendritic cells (DC). In the skin, DC-SIGN+ DC are exclusively located in the dermis. DC-SIGN binds to ICAM-3 on resting T cells, establishing DC-T cell contact and adaptive immunity. DC-SIGN is a high affinity receptor for HIV gp120, allowing HIV capture and transmission to CD4+ T cells. In addition to HIV, DC-SIGN is a receptor for a number of other viral and cellular pathogens including Mycobacterium Tuberculosis, and is a major player in microbial evasion of the immune system. (Geijtenbeek, T et al, Cell; 2000; 100: 587-597; van Kooyk Y et al, Nat. Rev. Immunol.; 2003; 3: 697-709)
• Synonyms: DCSIGN1, DCSIGN, DC-SIGN1, CLEC4L, Dendritic Cell Marker