DC SIGN (CD209) (Extracell. Dom.) Mouse Monoclonal Antibody [Clone ID: 128G8.43]

CAT#: DDX0201P-100

DC SIGN (CD209) (Extracell. Dom.) mouse monoclonal antibody, clone 128G8.43

Product Images

IHC staining of human lung frozen section with clone 128G8 (DX0201)

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  Frozen serial sections of human skin stained with 128G8

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  DC-SIGN like expression on human monocyte-derived DCs subsets

Specifications

Product Data

• Clone Name: 128G8.43
• Applications: FC, IHC, WB
• Recommended Dilution: Antigen uptake, FACS surface, ImmunoHistoChemistry frozen sections, Western Blot.
• Reactivities: Human, Sheep
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: human (GMCSF + TNF) DC subset.
• Specificity: Human (epitope in extracellular domain).
  Species cross-reactivity: Sheep.
• Formulation: Purified: 100 μg in 200 μl / 50 μg in 100 μl Tris-NaCl pH 8
  State: Purified
• Concentration: lot specific
• Purification: QMA Hyper D ion exchange chromatography
• Conjugation: Unconjugated
• Storage: -20°C. KEEP CONTENTS STERILE: no preservative.
  Purified antibodies: avoid repeated freeze/thaw cycles.
  Coupled antibodies: glycerol protects from freezing.
• Gene Name: CD209 molecule
• Database Link:
  Entrez Gene 30835 Human
  UniProt ID Q9NNX6
• Background: We have generated an antibody recognizing an antigen which has a tissular distribution and a size (western blot) identical to DC-SIGN. This antibody does not recognize DC-SIGN 1 expressed in HeLa cells. DCSIGN (“DC Specific, ICAM-3 Grabbing, Nonintegrin”) / CD209 is a type II membrane protein with an external mannose-binding C-type lectin domain, DC-SIGN is expressed by immature and mature dendritic cells (DC). In the skin, DC-SIGN+ DC are exclusively located in the dermis. DC-SIGN binds to ICAM-3 on resting T cells, establishing DC-T cell contact and adaptive immunity. DC-SIGN is a high affinity receptor for HIV gp120, allowing HIV capture and transmission to CD4+ T cells. In addition to HIV, DC-SIGN is a receptor for a number of other viral and cellular pathogens including Mycobacterium Tuberculosis, and is a major player in microbial evasion of the immune system.
  (Geijtenbeek, T and al, Cell; 2000; 100: 587-597; van Kooyk Y and al, Nat. Rev. Immunol.; 2003; 3: 697- 709)
• Synonyms: DCSIGN1, DCSIGN, DC-SIGN1, CLEC4L, Dendritic Cell Marker