DC SIGN (CD209) Mouse Monoclonal Antibody [Clone ID: 108C7.01]

CAT#: DDX0204A647-100

DC SIGN (CD209) mouse monoclonal antibody, clone 108C7.01


USD 530.00

2 Weeks*

Size
    • 100 ug

Product Images

Specifications

Product Data
Clone Name 108C7.01
Applications FC
Recommended Dilution DDX0204P-50 / DDX0204P-100 Purified: Surface flow cytometry, HIV gp120 binding studies.
DDX0204A488-50 / DDX0204A488-100 Alexa-fluor®488: Surface Flow cytometry.
DDX0204A546-50 / DDX0204A546-100 Alexa- fluor®546: Immunofluorescence.
DDX024A647-50 / DDX0204A647-100
 Alexa- fluor®647: Surface Flow cytometry
DDX0204B-50 / DDX0204B-100 Biotin (on request): 
 FACS surface, ImmunoHistoChemistry frozen sections. 
Usage recommendation: 

*This monoclonal antibody may be used between 5-20 μg/ml.
Reactivities Human
Host Mouse
Isotype IgG2b
Clonality Monoclonal
Immunogen HeLa cells stably transfected-with human DC-SIGN.
Specificity Human DC-SIGN.
Specied cross-reactivity: Human L-SIGN. Not tested on other species
Formulation Purified: 100 μg in 200μl / 50 μg in 100 μl Tris-NaCl pH 8
Coupled: 100 μg in 200 μl / 50 μg in 100 μl PBS 50% glycerol
Label: Alexa Fluor 647
Concentration lot specific
Conjugation Alexa Fluor 647
Storage -20°C. KEEP CONTENTS STERILE: no preservative.
Purified antibodies: avoid repeated freeze/thaw cycles.
Coupled antibodies: glycerol protects from freezing.
Gene Name CD209 molecule
Background DC-SIGN (“DC Specific, ICAM-3 Grabbing, Nonintegrin”) / CD209 and liver/lymph node-specific ICAM-3-grabbing nonintegrin (L-SIGN) (CD299/DC-SIGNR for DC-SIGN-related molecule; DC-SIGN2) are closely related genes that map to chromosome 19p13.3.Both genes encode a member of the C-type lectin family of type II transmembrane proteins. The two receptors are 77% identical at the amino acid level, have similar ligands. They are expressed in different tissues. DC-SIGN is expressed on dendritic cells and macrophages . L-SIGN is found in the endothelial cells of liver, lymph nodes, and placenta and is absent on DCs and macrophages. Both receptors have been shown to interact with ICAM-3 DC-SIGN is a high affinity receptor for HIV gp120, (Soilleux EJ. 2003, Clinical Science 104, 437-; Dakappagari N., et al. 2006, The J Immunol, 176, 426 ; Geijtenbeeck T.B., et al. 2000, Cell, 100, 575 ; Bashirova A. et al., 2001, J.Exp. Med.,193, 671) Antibodies have been selected with NIH3T3 transfected cells with either L-SIGN, or DC-SIGN.
Synonyms DCSIGN1, DCSIGN, DC-SIGN1, CLEC4L, Dendritic Cell Marker
Reference Data

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.