DC SIGN (CD209) Mouse Monoclonal Antibody [Clone ID: 108C7.01]

CAT#: DDX0204A647-100

DC SIGN (CD209) mouse monoclonal antibody, clone 108C7.01

Product Images

HIV-gp120 binding on DC-SIGN HeLa transfected cells. red: without blocking, blue: blocking activity

Specifications

Product Data

• Clone Name: 108C7.01
• Applications: FC
• Recommended Dilution: DDX0204P-50 / DDX0204P-100 Purified: Surface flow cytometry, HIV gp120 binding studies.
  DDX0204A488-50 / DDX0204A488-100 Alexa-fluor®488: Surface Flow cytometry.
  DDX0204A546-50 / DDX0204A546-100 Alexa- fluor®546: Immunofluorescence.
  DDX024A647-50 / DDX0204A647-100 Alexa- fluor®647: Surface Flow cytometry
  DDX0204B-50 / DDX0204B-100 Biotin (on request): FACS surface, ImmunoHistoChemistry frozen sections.
  Usage recommendation:
  *This monoclonal antibody may be used between 5-20 μg/ml.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: HeLa cells stably transfected-with human DC-SIGN.
• Specificity: Human DC-SIGN.
  Specied cross-reactivity: Human L-SIGN. Not tested on other species
• Formulation: Purified: 100 μg in 200μl / 50 μg in 100 μl Tris-NaCl pH 8
  Coupled: 100 μg in 200 μl / 50 μg in 100 μl PBS 50% glycerol
  Label: Alexa Fluor 647
• Concentration: lot specific
• Conjugation: Alexa Fluor 647
• Storage: -20°C. KEEP CONTENTS STERILE: no preservative.
  Purified antibodies: avoid repeated freeze/thaw cycles.
  Coupled antibodies: glycerol protects from freezing.
• Gene Name: CD209 molecule
• Database Link:
  Entrez Gene 30835 Human
  UniProt ID Q9NNX6
• Background: DC-SIGN (“DC Specific, ICAM-3 Grabbing, Nonintegrin”) / CD209 and liver/lymph node-specific ICAM-3-grabbing nonintegrin (L-SIGN) (CD299/DC-SIGNR for DC-SIGN-related molecule; DC-SIGN2) are closely related genes that map to chromosome 19p13.3.Both genes encode a member of the C-type lectin family of type II transmembrane proteins. The two receptors are 77% identical at the amino acid level, have similar ligands. They are expressed in different tissues. DC-SIGN is expressed on dendritic cells and macrophages . L-SIGN is found in the endothelial cells of liver, lymph nodes, and placenta and is absent on DCs and macrophages. Both receptors have been shown to interact with ICAM-3 DC-SIGN is a high affinity receptor for HIV gp120, (Soilleux EJ. 2003, Clinical Science 104, 437-; Dakappagari N., et al. 2006, The J Immunol, 176, 426 ; Geijtenbeeck T.B., et al. 2000, Cell, 100, 575 ; Bashirova A. et al., 2001, J.Exp. Med.,193, 671) Antibodies have been selected with NIH3T3 transfected cells with either L-SIGN, or DC-SIGN.
• Synonyms: DCSIGN1, DCSIGN, DC-SIGN1, CLEC4L, Dendritic Cell Marker