DC SIGN (CD209) Mouse Monoclonal Antibody [Clone ID: 120C8]

CAT#: DDX0208A488-100

DC SIGN (CD209) mouse monoclonal antibody, clone 120C8

Product Images

Canard B et al, Immunol Lett, 2011

Specifications

Product Data

• Clone Name: 120C8
• Applications: FC, IF
• Recommended Dilution: DDX0208P-50 / DDX0208P-100 purified: Surface Flow cytometry, Inhibition of HIVgp120 binding to DC-SIGN
  DDX0208A488-50 / DDX0208A488-100 Alexa-Fluor®488: Surface Flow cytometry , IF
  DDX0208A647-50 / DDX0208A647-100 Alexa-Fluor®647: Surface Flow cytometry.
  DDX0208B-50 DDX0208B-100 Biotin (on request): Surface Flow cytometry.
  Usage recommendation:
  *This monoclonal antibody may be used between 3-20 μg/ml.
  *Optimal dilution should be determined by each laboratory for each application.
  *Coupled antibody: to maintain RT before use.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: HeLa cells stably transfected-with human DC-SIGN.
• Specificity: Human (epitope in extracellular domain).
  Species cross-reactivity: no cross-reactivity with Human L-SIGN.
• Formulation: Purified: 100 μg in 200 μl / 50 μg in 100 μl Tris-NaCl pH 8.
  Coupled: 100 μg in 200 μl / 50 μg in 100 μl PBS 50% glycerol.
  Label: Alexa Fluor 488
• Concentration: lot specific
• Conjugation: Alexa Fluor 488
• Storage: -20°C. KEEP CONTENTS STERILE: no preservative.
  Purified antibodies: avoid repeated freeze/thaw cycles.
  Coupled antibodies: glycerol protects from freezing.
• Gene Name: CD209 molecule
• Database Link:
  Entrez Gene 30835 Human
  UniProt ID Q9NNX6
• Background: DC-SIGN (“DC Specific, ICAM-3 Grabbing, Nonintegrin”) / CD209 is a type II membrane protein with an external mannose-binding C-type lectin domain. DC-SIGN is expressed by immature and mature dendritic cells (DC). In the skin, DC-SIGN+ DC are exclusively located in the dermis. DC-SIGN binds to ICAM-3 on resting T cells, establishing DC-T cell contact and adaptive immunity. DC-SIGN is a high affinity receptor for HIV gp120, allowing HIV capture and transmission to CD4+ T cells. In addition to HIV, DC-SIGN is a receptor for a number of other viral and cellular pathogens including Mycobacterium tuberculosis, and is a major player in microbial evasion of the immune system.
  (Geijtenbeek, T and al, Cell, 2000; 100: 587-597; van Kooyk Y and al, Nat. Rev. Immunol., 2003; 3: 697-709).
• Synonyms: DCSIGN1, DCSIGN, DC-SIGN1, CLEC4L, Dendritic Cell Marker
• Note: Protocol: HIV-gp120 blocking protocol:
  HeLa and HeLa-DC-SIGN were incubated with 100μl of DC-SIGN antibody (3-10μg/ml) during 30’ at 37°C. Cells were washedwith culture medium during 5’ at 1600 rpm, and stained with biotin-gp120 (5μg/ml) during 1h at 37°C (Immunodiagnostics). Cells were washed with culture medium during 5’ at 1600 rpm followed by staining with PE-conjugated streptavidin (dilution 1/20) during 30’ at 4°C (Becton Dickinson). After a last washing, cells were analyzed by flow cytometry.