Peptidase 1 / DERP1 Human Monoclonal Antibody [Clone ID: H.AK5.C]

CAT#: DDX0212P-100

Peptidase 1 / DERP1 human monoclonal antibody, clone H.AK5.C

Product Images

Human monoclonal antibodies were tested in an indirect ELISA to detect Dermatophagoïdes Pteronyssinus antigens : crude extract (DPT) or recombinantDerp1or Derp2

Specifications

Product Data

• Clone Name: H.AK5.C
• Applications: ELISA, WB
• Recommended Dilution: ELISA, Western Blot, Biopanning.
  Usage recommendation:
  *This monoclonal antibody may be used between 1-20 μg/ml.
  *Optimal dilution should be determined by each laboratory for each application.
  *Coupled antibody: to maintain RT before use.
  
• Host: Human
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: EBV cell clones derived from PBMC of allergic patients to DPT.
• Specificity: allergen of the house-dust mites Dermatophagoides pteronyssinus Derp 1
• Formulation: 100 μg in 200 μl / 50 μg in 100 μl Tris-NaCl pH 8
  State: Purified
• Concentration: 0.5 mg/ml
• Purification: QMA Hyper D ion exchange chromatography
• Background: House-dust mite allergy belongs to the most common allergies worldwide which affect more than 50% of all allergic patients. House dust mite allergens play an important role in inducing IgE-mediated sensitization and the development of bronchial hyperresponsiveness and asthma, perennial rhinitis, and atopic dermatitis. Allergic patients’s sera (IgE) recognize the house-dust mite Dermatophagoides Pteronyssinus Derp 1. The key event in these reactions is the cross-linking of IgE antibodies on effector cells by allergens, which induces a release of biologically active mediators like histamine and leukotrienes leading to different symptoms of allergy. Studies in vivo have shown that the cysteine peptidase activity of group 1 house dust mite allergens contributes to their allergenicity.
  (Chapman MD, 1980; J. Immunol., 125: 587-592; Hales BJ, Thomas WR., 1997; Clin. Exp. Allergy, 27(8):868- 875; Doull IJ. et al, 1997; Allergy, 52,220; Custovic A, 1996; J. Allergy Clin. Immunol., 98: 64- 72; Szalai K. et al., 2008; Mol. Immunol. 45:5, 1308-17)
• Synonyms: Allergen Der p I