HLA-alpha1 Mouse Monoclonal Antibody [Clone ID: mAb90]

CAT#: DDX0250P-100

HLA-alpha1 mouse monoclonal antibody, clone mAb90

Product Images

mAb90 inhibits CD40-dependent B-cell proliferation. Tonsil B cells were cultured for 5 days on CD32-L cells in the presence of 1μg/ml anti-CD40 mAb with 1μg/ml of W6/32 or mAb90

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  mAb90 induces apoptosis of CD40-activated B cells. Cells were collected after 12 hours of treatment with the different mAbs and DNA from 2X104 cells was run on 2% agarose gel and stainedwith ethidiumbromide.

Specifications

Product Data

• Clone Name: mAb90
• Applications: ELISA, FC, FN
• Recommended Dilution: FACS, cell culture, ELISA capture (detecting antibody DDX0270).
  Usage recommendation:
  *This monoclonal antibody may be used between 1-20 μg/ml.
  *Optimal dilution should be determined by each laboratory for each application.
  *Coupled antibody: to maintain RT before use.
  
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Tonsillar B cells.
• Formulation: Purified: 100 μg in 200 μl / 50 μg in 100 μl Tris-NaCl pH 8
  State: Purified
• Concentration: 0.5 mg/ml
• Purification: QMA Hyper D ion exchange chromatography.
• Background: Antibodies to HLA class I α1 domain trigger apoptosis of activated T-cells and also control the growth/survival of human B lymphocytes. Addition of mAb 90 strongly inhibits the proliferation of CD40- activated total tonsillar B cells, purified naive, germinal center and memory B-cell subsets. mAb 90 binds to an epitope of the α-1 domain of HLA class I heavy chain induces apoptotic cell death of activated but not resting peripheral T lymphocytes.
  (Genestier L. et al., 1997 Blood, 90, 726-35; Genestier L. et al. 1997, Blood, 90, 3629-3639).