Influenza M1/HLA-A2 Complex (Extracell. Dom.) Mouse Monoclonal Antibody [Clone ID: 405H1.01]

CAT#: DDX0270A647-100

Influenza M1/HLA-A2 Complex (Extracell. Dom.) mouse monoclonal antibody, clone 405H1.01

Product Images

M1 loading on HLA.A2 transfected EL4 cells: Flow cytometry with clone 405H1.01

Specifications

Product Data

• Clone Name: 405H1.01
• Applications: FC
• Recommended Dilution: DDX0270P-50 / DDX0270P-100 Purified: Flow cytometry, ELISA.
  DDX0270A488-50 / DDX0270A488-100 Alexa-fluor®488: Flow cytometry.
  DDX0270A546-50 / DDX0270A546-100 Alexa-fluor®488: Flow cytometry..
  DDX0270A647-50 / DDX0270A647-100 Alexa-fluor®488: Flow cytometry.
  DDX0270B-50 / DDX0270B-100 Biotin: ELISA.
  
  Usage recommendation:
  *This monoclonal antibody may be used between 5-20 μg/ml.
  *Optimal dilution should be determined by each laboratory for each application.
  *Coupled antibody: to maintain RT before use.
  
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: T2 cell line charged in M1 peptide
• Specificity: Human extracellular/M1 HLA neuropilin-1(epitope in extracellular domain).
• Formulation: Purified: 100 μg in 200 μl / 50 μg in 100 μl Tris-NaCl pH 8.
  Coupled: 100 μg in 200 μl / 50 μg in 100 μl PBS 50% glycerol.
  Label: Alexa Fluor 647
• Concentration: 0.5 mg/ml
• Purification: QMA Hyper D ion exchange chromatography
• Conjugation: Alexa Fluor 647
• Background: In order to generate this mAb, the HLA.A2 heavy chain and human 2-m were produced in bacteria, purified and covalently bound to the immunodominant HLA.A2 restricted peptide 58-66 of the influenza virus matrix protein (M1p). Correct refolding was confirmed by staining specific CD8+ CTL clones with the biotinylated recombinant chimera that had been tetramerized with PE-streptavidin. Balb/c mice were immunized with the chimera and hybridomas were tested by a differential chimera-based ELISA containing M1-HLA.A2 versus HIVgag-HLA.A2 and empty HLA.A2 constructs. 405H1 mAb was found to be specific for M1-HLA.A2 with a Kd = 3,3.10^-10 M. 405H1 represents a valuable tool for studying the processing and direct or cross-presentation on MHC-I molecule.