Influenza M1/HLA-A2 Complex (Extracell. Dom.) Mouse Monoclonal Antibody [Clone ID: 405H1.01]

CAT#: DDX0270B-100

Influenza M1/HLA-A2 Complex (Extracell. Dom.) mouse monoclonal antibody, clone 405H1.01


USD 490.00

2 Weeks*

Size
    • 100 ug

Product Images

Specifications

Product Data
Clone Name 405H1.01
Applications ELISA
Recommended Dilution DDX0270P-50 / DDX0270P-100 Purified: Flow cytometry, ELISA.
DDX0270A488-50 / DDX0270A488-100 Alexa-fluor®488: Flow cytometry.
DDX0270A546-50 / DDX0270A546-100 Alexa-fluor®488: Flow cytometry.. 
DDX0270A647-50 / DDX0270A647-100 Alexa-fluor®488: Flow cytometry. 
DDX0270B-50 / DDX0270B-100 Biotin: ELISA.

Usage recommendation:

*This monoclonal antibody may be used between 5-20 μg/ml.
*Optimal dilution should be determined by each laboratory for each application.
*Coupled antibody: to maintain RT before use.
Reactivities Human
Host Mouse
Isotype IgG1
Clonality Monoclonal
Immunogen T2 cell line charged in M1 peptide
Specificity Human extracellular/M1 HLA neuropilin-1(epitope in extracellular domain).
Formulation Purified: 100 μg in 200 μl / 50 μg in 100 μl Tris-NaCl pH 8.
Coupled: 100 μg in 200 μl / 50 μg in 100 μl PBS 50% glycerol.
Label: Biotin
Concentration 0.5 mg/ml
Purification QMA Hyper D ion exchange chromatography
Conjugation Biotin
Background In order to generate this mAb, the HLA.A2 heavy chain and human 2-m were produced in bacteria, purified and covalently bound to the immunodominant HLA.A2 restricted peptide 58-66 of the influenza virus matrix protein (M1p). Correct refolding was confirmed by staining specific CD8+ CTL clones with the biotinylated recombinant chimera that had been tetramerized with PE-streptavidin. Balb/c mice were immunized with the chimera and hybridomas were tested by a differential chimera-based ELISA containing M1-HLA.A2 versus HIVgag-HLA.A2 and empty HLA.A2 constructs. 405H1 mAb was found to be specific for M1-HLA.A2 with a Kd = 3,3.10-10 M. 405H1 represents a valuable tool for studying the processing and direct or cross-presentation on MHC-I molecule.
Reference Data

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.