MMP12 Mouse Monoclonal Antibody [Clone ID: 603E6.22]

CAT#: DDX0280B-100

MMP12 mouse monoclonal antibody, clone 603E6.22

Product Images

Human lung tissue sections

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  IHC staining of human lung cancer frozen section with clone 603E6 (DX0280)

Specifications

Product Data

• Clone Name: 603E6.22
• Applications: IHC
• Recommended Dilution: DDX0280P-50 / DDX0280P-100 Purified: ELISA, ImmunoHistoChemistry frozen sections, Immunoprecipitation.
  DDX0280B-50 / DDX0280B-100 Purified: ImmunoHistoChemistry frozen sections.
  
  Usage recommendation:
  *This monoclonal antibody may be used between 5-20μg/ml.
  *Optimal dilution should be determined by each laboratory for each application.
  *Coupled antibody: to maintain RT before use.
  
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: recombinant HME
• Specificity: Human HME-MMP12 (epitope in proenzymatic region).
• Formulation: Purified: 100 μg in 200μl / 50 μg in 100 μl Tris-NaCl pH 8.
  Coupled: 100 μg in 200μl / 50 μg in 100 μl PBS 50% glycerol.
  Label: Biotin
• Concentration: lot specific
• Purification: QMA Hyper D ion exchange chromatography
• Conjugation: Biotin
• Storage: -20°C. KEEP CONTENTS STERILE: no preservative.
  Purified antibodies: avoid repeated freeze/thaw cycles.
  Coupled antibodies: glycerol protects from freezing.
• Gene Name: matrix metallopeptidase 12
• Database Link:
  Entrez Gene 4321 Human
  UniProt ID P39900
• Background: Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes. HME/MMP-12, also called metalloelastase, is reported only in few cells, including tissue macrophages and hypertrophic chondrocytes. MMP-12 is critical for invasion and destruction in pathologies such as aneurysm and emphysema. The predicted molecular mass of the HME proenzyme is 54 kDa. HME mRNA and protein were detected in human alveolar macrophages. Similar to murine macrophage metalloelastase, HME readily undergoes NH2- and COOH-terminal processing to a mature 22 kDa form. Both recombinant expressed in Escherichia Coli and native HME derived from human alveolar macrophage-conditioned media degraded insoluble elastin. HME is a unique human metalloproteinase that displays elastolytic activity and is expressed in alveolar macrophages; MMP12 mediates smoke-induced inflammation by releasing TNFa from macrophages, with subsequent endothelial activation, neutrophil influx, and proteolytic matrix breakdown caused by neutrophil-derived proteases. (Demets IK et al, 2006; Thorax, 61:196-201)
• Synonyms: Macrophage metalloelastase, HME, ME, Matrix metalloproteinase-12, MMP12, Macrophage elastase