CD39 (ENTPD1) Mouse Monoclonal Antibody [Clone ID: AC2.5]

CAT#: DDX0321A546-100

CD39 (ENTPD1) mouse monoclonal antibody, clone AC2.5

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Specifications

Product Data

• Clone Name: AC2.5
• Applications: IF
• Recommended Dilution: DDX0321P-50 DDX0321P-100 Purified: FACS surface, ImmunoHistoChemistry frozen sections, Immunoprecipitation, Western Blot.
  DDX032A488-50 DDX0321A488-100 AlexaFluor®488: FACS surface, Immunofluorescence.
  DDX0321A546-50 DDX0321A546-100 AlexaFluor®546: Immunofluorescence.
  DDX0321A647-50 DDX0321A647-100 AlexaFluor®647: Surface and Intracyto Flow cytometry
  DDX0321B-50 DDX0321B-100 Biotin: Western Blot, ImmunoHistoChemistry frozen sections.
  
  Usage recommendation:
  *This monoclonal antibody may be used between 5-20 μg/ml.
  *Optimal dilution should be determined by each laboratory for each application.
  *Coupled antibody: to maintain RT before use.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: EBV-transformed human B lymphoblastoid cell line.
• Specificity: Human CD39.
• Formulation: Purified: 100 μg in 200 μl / 50 μg in 100 μl Tris-NaCl pH 8.
  Coupled: 100 μg in 200μl / 50 μg in 100 μl PBS 50% glycerol.
  Label: Alexa Fluor 546
• Concentration: lot specific
• Purification: QMA Hyper D ion exchange chromatography
• Conjugation: Alexa Fluor 546
• Storage: -20°C. KEEP CONTENTS STERILE: no preservative.
  Purified antibodies: avoid repeated freeze/thaw cycles.
  Coupled antibodies: glycerol protects from freezing.
• Gene Name: ectonucleoside triphosphate diphosphohydrolase 1
• Database Link:
  Entrez Gene 953 Human
  UniProt ID P49961
• Background: CD39, also known as ENTPD1, is a 58KDa multi-pass membrane protein belonging to the GDA1/CD39 NTPase family. It is expressed primarily on activated lymphoid cells and can also be detected in endothelial tissues. The vascular isoform and the placental isoform II are present in both placenta and umbilical vein, whereas placental isoform I is present in placenta only. CD39 can hydrolyze both nucleoside triphosphates and diphosphates. AC2.5 was generated after mouse immunization with EBV-transformed B cells. (Rowe M et al, Int J Cancer. 1982 Apr 15;29(4):373- 81).
• Synonyms: NTPDase 1, Ecto-apyrase, ATPDase