Langerin (CD207) Mouse Monoclonal Antibody [Clone ID: 808E10.01]

CAT#: DDX0360A488-100

Langerin (CD207) mouse monoclonal antibody, clone 808E10.01

Product Images

IF staining of Langerin-transfected CHO cells with 808E10 (DDX0360)

Specifications

Product Data

• Clone Name: 808E10.01
• Applications: FC, IF
• Recommended Dilution: Surface Flow cytometry, IF
• Reactivities: Human, Mouse
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: human Langerin-transfected eukaryotic cells
• Specificity: Human Langerin (extracellular epitope).
  Species cross-reactivity: Mouse.
• Formulation: PBS 50% glycerol
  Label: Alexa Fluor 488
• Concentration: lot specific
• Purification: QMA Hyper D ion exchange chromatography
• Conjugation: Alexa Fluor 488
• Storage: -20°C. KEEP CONTENTS STERILE: no preservative.
  Purified antibodies: avoid repeated freeze/thaw cycles.
  Coupled antibodies: glycerol protects from freezing.
• Gene Name: CD207 molecule
• Database Link:
  Entrez Gene 50489 Human
  UniProt ID Q9UJ71
• Background: Langerin/CD207 is a transmembrane C-type lectin receptor (CLR) of epidermal and mucosal Langerhans cells (LCs) that induces Birbeck granule formation. Langerin features a single carbohydrate recognition domain (CRD) with mannose-type specificity in its extracellular portion. Langerin is unique among the CLRs in that it contains an intracellular domain with a proline-rich motif. Langerin expression has not been reported outside the DC system.
  (Valladeau J et al, 2002; J. Immunol., 168 : 782-779 ; Douillard P et al, 200; J. Invest. Dermatol., 125: 983–994).
• Synonyms: CLEC4K, Dendritic Cell Marker
• Note: Protocol: Langerin-gp120 blocking protocol:
  Mock- and Langerin-transfected CHO cells are incubated with biotin-labelled gp160 (5μg/ml) and clone 808E10.01 at (1 to 10μg/ml) in PBS 45 min. at RT. Cells were washed in PBS 5min at 1600rpm, and stained with Streptavidin-PE 30 min at 4°C (DAKO). A last PBS wash is performed before Flow cytometry analysis.