Langerin (CD207) Mouse Monoclonal Antibody [Clone ID: 808E10.01]

CAT#: DDX0360P-100

Langerin (CD207) mouse monoclonal antibody, clone 808E10.01

Product Images

IF staining of Langerin-transfected CHO cells with 808E10 (DDX0360)

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  Human skin frozen section: Langerhans cells staining with 808E10.01-HRP

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  Blocking HIV-gp120 (808E10.01 anti-langerin clone)

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  Non -blocking anti-langerin clone

Specifications

Product Data

• Clone Name: 808E10.01
• Applications: FC, FN, IHC
• Recommended Dilution: Flow cytometry, blocks HIVgp120, IHC.
  
  Usage recommendation:
  *This monoclonal antibody may be used between 5-20 μg/ml.
  *Optimal dilution should be determined by each laboratory for each application.
  *Coupled antibody: to maintain RT before use.
  
• Reactivities: Human, Mouse
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human Langerin-transfected eukaryotic cells
• Specificity: Human Langerin (extracellular epitope).
  Species cross-reactivity: Mouse.
• Formulation: Tris-NaCl pH 8
  State: Purified
• Concentration: lot specific
• Purification: QMA Hyper D ion exchange chromatography
• Conjugation: Unconjugated
• Storage: -20°C. KEEP CONTENTS STERILE: no preservative.
  Purified antibodies: avoid repeated freeze/thaw cycles.
  Coupled antibodies: glycerol protects from freezing.
• Gene Name: CD207 molecule
• Database Link:
  Entrez Gene 50489 Human
  UniProt ID Q9UJ71
• Background: Langerin/CD207 is a transmembrane C-type lectin receptor (CLR) of epidermal and mucosal Langerhans cells (LCs) that induces Birbeck granule formation. Langerin features a single carbohydrate recognition domain (CRD) with mannose-type specificity in its extracellular portion. Langerin is unique among the CLRs in that it contains an intracellular domain with a proline-rich motif. Langerin expression has not been reported outside the DC system.
  (Valladeau J et al, 2002; J. Immunol., 168 : 782-779 ; Douillard P et al, 200; J. Invest. Dermatol., 125: 983–994)
• Synonyms: CLEC4K, Dendritic Cell Marker
• Note: Protocol: Langerin-gp120 blocking protocol:
  Mock- and Langerin-transfected CHO cells are incubated with biotin-labelled gp160 (5μg/ml) and clone 808E10.01 at (1 to 10μg/ml) in PBS 45 min. at RT. Cells were washed in PBS 5min at 1600rpm, and stained with Streptavidin-PE 30 min at 4°C (DAKO). A last PBS wash is performed before Flow cytometry analysis.