Langerin (CD207) Mouse Monoclonal Antibody [Clone ID: DCGM4/122D5.03]
Specifications
Product Data | |
Clone Name | DCGM4/122D5.03 |
Applications | FC, IHC, WB |
Recommended Dilution | DDX0363P-50 DDX0363P-100 Purified: Internalization, Flow cytometry, IHC. DDX0363A488-50 DDX0363A488-100 Alexa-fluor®488: Flow cytometry, IF. DDX0363A546-50 DDX0363A546-100 Alexa-fluor®546: IF. DDX0363A647-50 DDX0363A647-100 Alexa- fluor®647: Flow cytometry. DDX0363B-50 DDX0363B-100 Biotin: Western blot. Usage recommendation: *This monoclonal antibody may be used between 5-20 μg/ml. *Optimal dilution should be determined y each laboratory for each application. *Coupled antibody: to maintain RT before using. |
Reactivities | Canine, Human |
Host | Mouse |
Isotype | IgG1 |
Clonality | Monoclonal |
Immunogen | In vitro-derived human DCs from CD34+ cells in GM-CSF+TNFα |
Specificity | Human Langerin (extracellular epitope in Carbohydrate Recognition domain). Species cross-reactivity: Dog. |
Formulation | Purified: 100 μg in 200μl / 50 μg in 100 μl Tris-NaCl pH 8. Coupled: 100 μg in 200μl / 50 μg in 100 μl PBS 50% glycerol. Label: Biotin |
Concentration | lot specific |
Purification | QMA Hyper D ion exchange chromatography |
Conjugation | Biotin |
Storage | -20°C. KEEP CONTENTS STERILE: no preservative. Purified antibodies: avoid repeated freeze/thaw cycles. Coupled antibodies: glycerol protects from freezing. |
Gene Name | CD207 molecule |
Database Link | |
Background | Langerin/CD207 is a transmembrane C-type lectin receptor (CLR) of epidermal and mucosal Langerhans cells (LCs) that induces Birbeck’s granule formation. Langerin features a single carbohydrate recognition domain (CRD) with mannose-type specificity in its extracellular portion. Langerin is unique among the CLRs in that it contains an intracellular domain with a proline-rich motif. Langerin expression has not been reported outside the DC system. (Valladeau J et al, 1999, Eur.J.Immunol., 29:2695-2704; Valladeau J et al, 2000 Immunity, 12 : 71-81; Kashihara M et al, 1986, J.Invest.Derm., 87 :602-607 ; Ito T et al, 1999, J.Immunol., 163 :1409-1419 ;Saeland S & Valladeau J, CD207 (Langerin) Workshop reports 2002, Leukocyte-Typing VII, White Cell Diff Antigens, D. Mason et al, Eds, Oxford University Press:306-307) |
Synonyms | CLEC4K, Dendritic Cell Marker |
Reference Data |
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complexities in the preparation of your product. International customers may expect an additional 1-2 weeks
in shipping.