Langerin (CD207) Mouse Monoclonal Antibody [Clone ID: 306G9.01/HD24]

CAT#: DDX0368A546-100

Langerin (CD207) mouse monoclonal antibody, clone 306G9.01/HD24

Product Images

IHC staining of human skin frozen section with clone 306G9 (DX0368)

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Specifications

Product Data

• Clone Name: 306G9.01/HD24
• Applications: IF
• Recommended Dilution: Flow cytometry, IF, IHC.
  
  Usage recommendation:
  *This monoclonal antibody may be used between 5-20 μg/ml
  *Optimal dilution should be determined by each laboratory for each application.
  *Coupled antibody: to maintain RT before using.
  
• Reactivities: Human, Mouse, Porcine, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Mouse langerin–transfected COP5 cells.
• Specificity: Mouse and Human Langerin (intracellular epitope).
  Species cross- reactivity: Human, Rat, Swine.
• Formulation: Purified: 100 μg in 200μl / 50 μg in 100 μl Tris-NaCl pH 8.
  Coupled: 100 μg in 200μl / 50 μg in 100 μl PBS 50% glycerol.
  Label: Alexa Fluor 546
• Concentration: lot specific
• Purification: QMA Hyper D ion exchange chromatography
• Conjugation: Alexa Fluor 546
• Storage: -20°C. KEEP CONTENTS STERILE: no preservative.
  Purified antibodies: avoid repeated freeze/thaw cycles.
  Coupled antibodies: glycerol protects from freezing.
• Gene Name: CD207 molecule
• Database Link:
  Entrez Gene 50489 Human
  UniProt ID Q9UJ71
• Background: Langerin/CD207 is a transmembrane C-type lectin receptor (CLR) of epidermal and mucosal Langerhans cells (LCs) that induces Birbeck granule formation. Langerin features a single carbohydrate recognition domain (CRD) with mannose-type specificity in its extracellular portion. Langerin is unique among the CLRs in that it contains an intracellular domain with a proline-rich motif. Langerin expression has not been reported outside the DC system. In mouse, clone 306G9 stains lymph node dendritic cells (Henri et al, 2001, J. Immunol, 167: 741-748) (Valladeau J. et al, 2002 J Immunol, 168: 782).
• Synonyms: CLEC4K, Dendritic Cell Marker