Cd207 Mouse Monoclonal Antibody [Clone ID: 205C1]

CAT#: DDX0370A647-100

Cd207 mouse monoclonal antibody, clone 205C1

Product Images

IF on acetone-fixed ear epidermal sheet

• 

  Freshly isolated CD11c+ Langerhans cells.

Specifications

Product Data

• Clone Name: 205C1
• Applications: FC, IF
• Recommended Dilution: FACS, IF (Douillard P et al, 2005 J. Invest. Dermatol, 125: 983–994).
  
  Usage recommendation:
  *This monoclonal antibody may be used between 5-20 μg/ml.
  *Optimal dilution should be determined by each laboratory for each application.
  *Coupled antibody: to maintain RT before using.
• Reactivities: Mouse
• Host: Mouse
• Isotype: IgM
• Clonality: Monoclonal
• Immunogen: Mouse ear crawl-out Langerhans cells.
• Specificity: Mouse Langerin.
• Formulation: Purified: 100 μg in 200μl / 50 μg in 100 μl Tris-NaCl pH 8.
  Coupled: 100 μg in 200μl / 50 μg in 100 μl PBS 50% glycerol.
  Label: Alexa Fluor 647
• Concentration: lot specific
• Purification: Hyper-D ion exchange chromatography
• Conjugation: Alexa Fluor 647
• Storage: -20°C. KEEP CONTENTS STERILE: no preservative.
  Purified antibodies: avoid repeated freeze/thaw cycles.
  Coupled antibodies: glycerol protects from freezing.
• Gene Name: CD207 antigen
• Database Link:
  Entrez Gene 246278 Mouse
  UniProt ID Q8VBX4
• Background: Langerin/CD207 is a transmembrane C-type lectin receptor (CLR) of epidermal and mucosal Langerhans cells (LCs) that induces Birbeck’s granule formation. Langerin features a single carbohydrate recognition domain (CRD) with mannose-type specificity in its extracellular portion. Langerin is unique among the CLRs in that it contains an intracellular domain with a proline-rich motif. Langerin expression has not been reported outside the DC system. (Valladeau J et al, 2002 J Immunol, 168 : 782-792) (Douillard P et al, 2005 J Invest Dermatol, 125: 983–994).
• Synonyms: CLEC4K, Dendritic Cell Marker