Mannose Receptor (MRC1) (Extracell. Dom.) Mouse Monoclonal Antibody [Clone ID: 122D2.08]

CAT#: DDX0380P-100

Mannose Receptor (MRC1) (Extracell. Dom.) mouse monoclonal antibody, clone 122D2.08


USD 440.00

2 Weeks*

Size
    • 100 ug

Specifications

Product Data
Clone Name 122D2.08
Applications FC, FN, IHC, IP, NEUT, WB
Recommended Dilution Surface Flow Cytometry, WB, IHC, IP, internalization, inhibition of antibody binding by mannan, inhibition of dextran-fitc uptake by mannose-R expressing cells
Reactivities Human, Porcine, Sheep
Host Mouse
Isotype IgG1
Clonality Monoclonal
Immunogen human (GM+TNF) DC subset
Specificity human mannose receptor (extracellular part).
Species cross-reactivity: swine, sheep
Formulation Purified: Tris-NaCl pH 8.
Coupled: PBS 50% glycerol (on request).
State: Purified
Concentration lot specific
Conjugation Unconjugated
Storage -20°C. KEEP CONTENTS STERILE: no preservative.
Purified antibodies: avoid repeated freeze/thaw cycles.
Coupled antibodies: glycerol protects from freezing.
Gene Name mannose receptor, C type 1
Background The mannose receptor (MR) /CD206 is a 175 kDa C-type lectin of type I, with 8 carbohydrate recognition domains (CRD) belonging to the pattern recognition receptors. It is an endocytic receptor of macrophages and endothelial cell subsets whose natural ligands include both self glycoproteins and microbial glycans. CD206 is predominantly expressed in tissue macrophages, myeloid dendritic cells, liver, skin, lymphoid organ and tumor. In humans, the MMR has been detected in cells located within the dermis, lamina propria, and T cell areas of the tonsil, in inflammatory epidermal DCs of atopic dermatitis patients, and in cells lining venous sinuses in the spleen. CD206 is also expressed by human CD14+ -derived DCs. mAb 122D2.08 was obtained after mice immunization with human dendritic cells. It recognizes large proteins (160-200kDa), and its binding to in vitro-generated DC is inhibited by cell preincubation with mannan, suggesting that 122D.08 is directed against the macrophage mannose receptor. The uptake of mannosylated proteins (expressed on bacteria and fungi) is important for the biologic function of DC. mAb 122D2.08 positively stains swine and sheep tissue sections (Schwartz-Cornil I, pers.communication) (Sallusto F. et al., 1995, J. Exp. Med., 182, 389-40; Wollenberg A. et al.,J Invest Dermatol, 2002, 119, 5, 1096-102 ; Marquet F et al, PLoS ONE, vol 6 (1) e16320).
Synonyms Macrophage mannose receptor, CLEC13D, CLEC13DL, MRC1L1
Reference Data

{0} Product Review(s)

0 Product Review(s) Submit review

Be the first one to submit a review

Product Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.