Mannose Receptor (MRC1) (Extracell. Dom.) Mouse Monoclonal Antibody [Clone ID: 122D2.08]

CAT#: DDX0380P-100

Mannose Receptor (MRC1) (Extracell. Dom.) mouse monoclonal antibody, clone 122D2.08

Product Images

In situ staining of swine skin DCS

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  IHC on human skin cryosection

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  FACS staining of swine DC in skin and afferent lymph

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  IHC staining of human lung frozen section with clone 122D2 (DX0380)

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  FACS staining of human in vitro generated DCs

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Specifications

Product Data

• Clone Name: 122D2.08
• Applications: FC, FN, IHC, IP, NEUT, WB
• Recommended Dilution: Surface Flow Cytometry, WB, IHC, IP, internalization, inhibition of antibody binding by mannan, inhibition of dextran-fitc uptake by mannose-R expressing cells
• Reactivities: Human, Porcine, Sheep
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: human (GM+TNF) DC subset
• Specificity: human mannose receptor (extracellular part).
  Species cross-reactivity: swine, sheep
• Formulation: Purified: Tris-NaCl pH 8.
  Coupled: PBS 50% glycerol (on request).
  State: Purified
• Concentration: lot specific
• Conjugation: Unconjugated
• Storage: -20°C. KEEP CONTENTS STERILE: no preservative.
  Purified antibodies: avoid repeated freeze/thaw cycles.
  Coupled antibodies: glycerol protects from freezing.
• Gene Name: mannose receptor, C type 1
• Database Link:
  Entrez Gene 4360 Human
  UniProt ID P22897
• Background: The mannose receptor (MR) /CD206 is a 175 kDa C-type lectin of type I, with 8 carbohydrate recognition domains (CRD) belonging to the pattern recognition receptors. It is an endocytic receptor of macrophages and endothelial cell subsets whose natural ligands include both self glycoproteins and microbial glycans. CD206 is predominantly expressed in tissue macrophages, myeloid dendritic cells, liver, skin, lymphoid organ and tumor. In humans, the MMR has been detected in cells located within the dermis, lamina propria, and T cell areas of the tonsil, in inflammatory epidermal DCs of atopic dermatitis patients, and in cells lining venous sinuses in the spleen. CD206 is also expressed by human CD14+ -derived DCs. mAb 122D2.08 was obtained after mice immunization with human dendritic cells. It recognizes large proteins (160-200kDa), and its binding to in vitro-generated DC is inhibited by cell preincubation with mannan, suggesting that 122D.08 is directed against the macrophage mannose receptor. The uptake of mannosylated proteins (expressed on bacteria and fungi) is important for the biologic function of DC. mAb 122D2.08 positively stains swine and sheep tissue sections (Schwartz-Cornil I, pers.communication) (Sallusto F. et al., 1995, J. Exp. Med., 182, 389-40; Wollenberg A. et al.,J Invest Dermatol, 2002, 119, 5, 1096-102 ; Marquet F et al, PLoS ONE, vol 6 (1) e16320).
• Synonyms: Macrophage mannose receptor, CLEC13D, CLEC13DL, MRC1L1