MIP3 alpha / CCL20 Mouse Monoclonal Antibody [Clone ID: 206D9.05]

CAT#: DDX0420-HRPO-100

MIP3 alpha / CCL20 mouse monoclonal antibody, clone 206D9.05

Product Images

IHC staining of human tonsil frozen section with clone 206D9 (DX0420)

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  Migration blocking of CD1a+LC precursors with 206D9.05

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  IHC staining of human tonsil frozen section with clone 206D9 (DX0420)

Specifications

Product Data

• Clone Name: 206D9.05
• Applications: ELISA
• Recommended Dilution: DDX0420P-50 DDX0420P-100 Purified: Blocking cell migration, ImmunoHistoChemistry frozen sections, ImmunoHistoChemistry paraffin sections, Western Blot.
  DDX0420HRPO-50 DDX0420HRPO-100 HRPO: ELISA detection (with 319F6.06 clone).
  
  Usage recommendation:
  *This monoclonal antibody may be used between 5-20 μg/ml.
  *Optimal dilution should be determined by each laboratory for each application.
  *Coupled antibody: to maintain RT before using.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant MIP3α in eukaryotic cells.
• Specificity: Human MIP-3α.
• Formulation: Purified: 100 μg in 200μl / 50 μg in 100 μl Tris-NaCl pH 8.
  Coupled: 100 μg in 200μl / 50 μg in 100 μl PBS 50% glycerol.
  Label: HRP
• Concentration: 0.5 mg/ml
• Purification: QMA Hyper D ion exchange chromatography
• Conjugation: HRP
• Background: Macrophage inflammatory protein (MIP)-3alpha/ CCL20 is a CC-type chemokine mapped to chromosome 2 in humans. Langerhans cells (LCs) represent a unique population of DCs colonizing epithelium. MIP-3alpha plays a central role in LC precursor recruitment into the epithelium during inflammation. (a) Among DC populations, MIP-3alpha is the most potent chemokine inducing the selective migration of in vitrogenerated CD34(+) hematopoietic progenitor cell-derived LC precursors and skin LCs in accordance with the restricted MIP-3alpha receptor (CC chemokine receptor 6) expression by these cells. (b) MIP-3alpha is mainly produced by epithelial cells, and the migration of LC precursors induced by the supernatant of activated skin keratinocytes is blocked with an antibody against MIP-3alpha. (c) In vivo, MIP-3alpha is selectively produced at sites of inflammation as illustrated in tonsils and lesional psoriatic skin where MIP- 3alpha upregulation appear associated with an increase in LC turnover. (d) The secretion of MIP-3alpha is strongly upregulated by cells of epithelial origin after inflammatory stimuli (interleukin 1beta plus tumor necrosis factor alpha) or T cell signals.(Dieu-Nosjean et al, J;Exp.med, 2000 192: 705-18;Homey B. et al, 2000,J.Immunol.164:6621-32).
• Synonyms: CCL20, LARC, MIP3A, MIP-3 alpha, C-C motif chemokine 20, Small-inducible cytokine A20, SCYA20, Exodus-1, Dendritic Cell Marker, DC marker