MIP3 alpha / CCL20 Mouse Monoclonal Antibody [Clone ID: 308B7.06]

CAT#: DDX0431B-100

MIP3 alpha / CCL20 mouse monoclonal antibody, clone 308B7.06

Product Images

IHC staining of human tonsil frozen section with clone 308B7 (DX0431)

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  Paraffin Bouin human tonsil section stained with 308B7.06-HRP.

Specifications

Product Data

• Clone Name: 308B7.06
• Applications: IHC
• Recommended Dilution: DDX0431P-50 DDX0431P-100: Purified: ImmunoHistoChemistry frozen sections, ImmunoHistoChemistry paraffin sections.
  DDX0431B-50 DDX0431B-100: Biotin: ImmunoHistoChemistry frozen sections, ImmunoHistoChemistry paraffin sections.
  
  Usage recommendation: *This monoclonal antibody may be used between 5-20 μg/ml.
  *Optimal dilution should be determined by each laboratory for each application.
  *Coupled antibody: to maintain RT before using.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant MIP3α in eukaryotic cells.
• Specificity: Human MIP-3α
• Formulation: Purified: 100 μg in 200μl / 50 μg in 100 μl Tris-NaCl pH 8.
  Coupled: 100 μg in 200 μl / 50 μg in 100 μl PBS 50% glycerol.
  Label: Biotin
• Concentration: 0.5 mg/ml
• Purification: QMA Hyper D ion exchange chromatography
• Conjugation: Biotin
• Background: Macrophage inflammatory protein (MIP)-3α/ CCL20 is a CC-type chemokine mapped to chromosome 2 in humans. Langerhans cells (LCs) represent a unique population of DCs colonizing epithelium. MIP-3α plays a central role in LC precursor recruitment into the epithelium during inflammation. (a) Among DC populations, MIP-3α is the most potent chemokine inducing the selective migration of in vitro-generated CD34+ hematopoietic progenitor cell-derived LC precursors and skin LCs in accordance with the restricted MIP-3α receptor (CC chemokine receptor 6) expression by these cells. (b) MIP-3α is mainly produced by epithelial cells, and the migration of LC precursors induced by the supernatant of activated skin keratinocytes is blocked with an antibody against MIP-3α. (c) In vivo, MIP-3α is selectively produced at sites of inflammation as illustrated in tonsils and lesional psoriatic skin where MIP-3α upregulation appears associated with an increase in LC turnover. (d) The secretion of MIP-3α is strongly upregulated by cells of epithelial origin after inflammatory stimuli (interleukin 1β plus TNFα) or T cell signals. (Dieu-Nosjeβan et al, J;Exp.med, 2000 192: 705-18)
• Synonyms: CCL20, LARC, MIP3A, MIP-3 alpha, C-C motif chemokine 20, Small-inducible cytokine A20, SCYA20, Exodus-1, Dendritic Cell Marker, DC marker