SEMA6A Mouse Monoclonal Antibody [Clone ID: 104B3]

CAT#: DDX0462A647-100

SEMA6A mouse monoclonal antibody, clone 104B3

Product Images

• 

  IHC staining of Langerhans histiocytosis frozen section with clone 104B3 (DDX0462)

Specifications

Product Data

• Clone Name: 104B3
• Applications: FC, IF
• Recommended Dilution: DDX0462P-50 / DDX0462P-100 Purified: IHC.
  DDX0462A488-50 / DDX0462A488-100 Alexa-fluor®488: IF, Flow cytometry
  DDX0462A546-50 / DDX0462A546-100 Alexa-fluor®546: IF
  DDX0462A647-50 / DDX0462A647-100 Alexa- fluor®647: (on request) IHC, Flow cytometry.
  
  Usage recommendation:
  *This monoclonal antibody may be used between 5-20 μg/ml.
  *Optimal dilution should be determined by each laboratory for each application.
  *Coupled antibody: to maintain RT before using.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: human recombinant SEMA-6A
• Specificity: Human SEMA-6A (epitope in extracellular domain).
• Formulation: Purified: 100 μg in 200μl / 50 μg in 100 μl Tris-NaCl pH 8.
  Coupled: 100 μg in 200μl / 50 μg in 100 μl PBS 50% glycerol.
  Label: Alexa Fluor 647
• Concentration: lot specific
• Purification: QMA Hyper D ion exchange chromatography
• Conjugation: Alexa Fluor 647
• Storage: -20°C. KEEP CONTENTS STERILE: no preservative.
  Purified antibodies: avoid repeated freeze/thaw cycles.
  Coupled antibodies: glycerol protects from freezing.
• Gene Name: semaphorin 6A
• Database Link:
  Entrez Gene 57556 Human
  UniProt ID Q9H2E6
• Background: Semaphorins represent a large family composed of 19 secreted, GPI-linked or transmembrane members divided into 5 classes (3 to 7) and characterized by the presence of a 500 amino acid Sema domain in the Nterminus. Initially described for their role in axon guidance and apoptosis during neuronal development, some semaphorins like the class 4 regulate immune functions and are involved in organogenesis and angiogenesis. The transmembrane class 6 semaphorins include 4 members (SEMA-6A to 6D). SEMA-6A features an intracellular binding site for regulatory Evl protein. In the immune system, SEMA6A protein is restricted in vitro and in vivo to an activation status of Langerhans cells. (Gautier G et al., 2006 Am J Pathol., 168: 453-65).
• Synonyms: Semaphorin-6A, Semaphorin VIA, Sema VIA, Semaphorin-6A-1, SEMA6A-1, SEMAQ, KIAA1368