CD283 / TLR3 Mouse Monoclonal Antibody [Clone ID: 619F7.06]

CAT#: DDX0470B-100

CD283 / TLR3 mouse monoclonal antibody, clone 619F7.06

Product Images

IHC staining of canine skin paraffin section with clone 619F7 (DX0470)

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  IF staining of human tonsil with 619F7 (DDX0470)

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  Western-blot analysis of human (h) and canine (c) PBMC and monocytes with 619F7.06

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  Intracellular FACS staining of human breast carcinoma CAMA-1 cell line with 619F7.06

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  Intracellular FACS staining of human monocyte-derivedDCs and human EBV B cells with 619F7.06

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  Intracellular FACS staining of canine blood cells with 619F7.06

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Specifications

Product Data

• Clone Name: 619F7.06
• Applications: WB
• Recommended Dilution: DDX0470P-50 / DDX0470P-100 Purified: FACS intracellular, ImmunoHistoChemistry frozen sections, ImmunoHistoChemistry paraffin sections, Western Blot.
  DDX0470A488-50 / DDX0470A488-100 Alexa-fluor®488: FACS intracellular, ImmunoFluorescence.
  DDX0470A546-50 / DDX0470A546-100 Alexa-fluor®546: ImmunoFluorescence.
  DDX0470A647-50 / DDX0470A647-100 Alexa- fluor®647: FACS intracellular.
  DDX0470B-50 / DDX0470B-100 Biotin: Western Blot.
  
  
  Usage recommendation:
  *This monoclonal antibody may be used between 5-20 μg/ml.
  *Optimal dilution should be determined by each laboratory for each application.
  *Coupled antibody: to maintain RT before using.
• Reactivities: Canine, Human, Mouse, Rat
• Host: Mouse
• Isotype: IgM
• Clonality: Monoclonal
• Immunogen: Human recombinant TLR3 in eukaryotic cells (pUNO-hTLR3)
• Specificity: Human TLR3 (intracytoplasmic).
  Species cross- reactivity: Rat, Dog, Mouse.
• Formulation: Purified: 100 μg in 200μl / 50 μg in 100 μl Tris-NaCl pH 8.
  Coupled: 100 μg in 200μl / 50 μg in 100 μl PBS 50% glycerol.
  Label: Biotin
• Concentration: 0.5 mg/ml
• Purification: QMA Hyper D ion exchange chromatography
• Conjugation: Biotin
• Background: The Toll-like receptor (TLR) family has recently emerged as key for sensing microbial infections and triggering inflammatory and innate responses. TLR share structural and functional homologies, as they are all able to detect micro-organisms through direct recognition of conserved Pathogen Associated Molecular Patterns (PAMPs) such as bacterial LPS or viral dsRNA. Ligand recognition is mediated through the Cterminal Leucine Rich Repeat region, while signalling involves the N-terminal Toll/Interleukin-1 Receptor (TIR) domain. TLR3 has been identified as a receptor for dsRNA. TLR3 is the only TLR that signals exclusively through the TIR-containing adaptor TRIF (TIR-containing adaptor inducing IFNβ), which activates the transcription factors IRF3 and NFκB, and triggers type I interferon secretion. TLR3 is expressed in various cell types, such as fibroblasts, dendritic cells (DCs), or NK cells. While TLR3 is expressed at the cell surface in fibroblasts, it is mostly found in intracytoplasmic vesicles in DCs. (Akira S et al, 2004, Nat Rev Immunol, 4: 499-511)
• Synonyms: Toll-like receptor 3