TLR10 Mouse Monoclonal Antibody [Clone ID: 25F9]

CAT#: DDX0490P-100

TLR10 mouse monoclonal antibody, clone 25F9

Product Images

IF staining of hTLR10-transfected COP5 cells with 25F9 (DDX0490)

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  Immunofluorescence staining with 25F9

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  Intracellular FACS staining with 25F9

Specifications

Product Data

• Clone Name: 25F9
• Applications: FC, IHC
• Recommended Dilution: DDX0490P-50 / DDX0490P-100 Purified: FACS intracellular, ImmunoHistoChemistry frozen sections.
  DDX0490A490-50 / DDX0490A488-100 Alexa-fluor®488 (on request): FACS intracellular, ImmunoFluorescence.
  DDX0490A546-50 / DDX0490A546-100 Alexa-fluor®546 (on request): FACS intracellular, ImmunoFluorescence.
  DDX0490A647-50 / DDX0490A647-100 Alexa-fluor®647(on request): FACS intracellular.
  
  Usage recommendation:
  *This monoclonal antibody may be used between 1-10 μg/ml.
  *Optimal dilution should be determined by each laboratory for each application.
  *Coupled antibody: to maintain RT before using.
  
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: rhTLR10-transfected eukaryotic cells.
• Specificity: Human TLR10.
• Formulation: Purified: 100 μg in 200μl / 50 μg in 100 μl Tris-NaCl pH 8.
  Coupled: 100 μg in 200μl / 50 μg in 100 μl Tris PBS 50% glycerol.
  State: Purified
• Concentration: lot specific
• Conjugation: Unconjugated
• Storage: -20°C. KEEP CONTENTS STERILE: no preservative.
  Purified antibodies: avoid repeated freeze/thaw cycles.
  Coupled antibodies: glycerol protects from freezing.
• Gene Name: toll like receptor 10
• Database Link:
  Entrez Gene 81793 Human
  UniProt ID Q9BXR5
• Background: The Toll-Like Receptors (TLR) is a family of germline-encoded proteins, composed of C-terminal leucinerich repeats (LRR), and an N-terminal Toll/Interleukin-1 Receptor (TIR) domain (1). In humans, 10 TLRs, sharing high sequence homology, have been identified. TLRs are critical for the detection of pathogenassociated molecular patterns (PAMPs) by the innate immune system. LRR recognize PAMPs, and signal transduction events, initiated by the TIR domain, lead to activation of the transcription factors such as AP-1, IRFs and NFkB, and therefore expression of proinflammatory cytokines and costimulatory molecules. TLR10 mRNA is detected in spleen, tonsils, lymph nodes, bone marrow and PBLs. Human TLR10 is an orphan member of the TLR family. Genomic studies indicate that TLR10 is in a locus that also contains TLR1 and TLR6, two receptors known to function as coreceptors for TLR2.
  (Akira, S et al, Nat Rev Immunol ,2004 4:499-511 ; Flacher V et al, J.Immunol, 2006,177, 959-67 ; Hasan U et al, J.Immunol, 2005, 174, 2942-50).
• Synonyms: Toll-like receptor 10, UNQ315/PRO358