TLR7 Mouse Monoclonal Antibody [Clone ID: 66H3]

CAT#: DDX0500P-100

TLR7 mouse monoclonal antibody, clone 66H3

Product Images

IHC staining of human FFPE tonsil section (Vermi William courtesy)

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  huTLR7-transfected 293 cells stained with 66H3

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  IHC staining of human tonsil cryosection with 66H3

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  huTLR7-transfected 293 cells stained with 66H3 (DDX0500)

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  Intracellular FACS staining of a canine cell line with 66H3

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  Intracellular FACS staining of human PBMC with 66H3

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Specifications

Product Data

• Clone Name: 66H3
• Applications: FC, IHC
• Recommended Dilution: DDX0500P-50 / DDX0500P-100 Purified: FACS intracellular, ImmunoHistoChemistry frozen sections, ImmunoHistoChemistry paraffin sections.
  DDX0480A488-50 / DDX0480A488-100 Alexa-fluor®488: FACS intracellular, ImmunoFluorescence.
  DDX0500A546-50 / DDX0500A546-100 Alexa-fluor®546: FACS intracellular, ImmunoFluorescence.
  DDX0500A647-50 / DDX0500A647-100 Alexa-fluor®647: FACS intracellular, ImmunoFluorescence.
  DDX0500B-50 / DDX0500B-100 Biotin: ImmunoHistoChemistry frozen sections, ImmunoHistoChemistry paraffin sections.
  
  Usage recommendation:
  *This monoclonal antibody may be used between 5-20 μg/ml.
  *Optimal dilution should be determined by each laboratory for each application.
  *Coupled antibody: to maintain RT before using.
  
• Reactivities: Canine, Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant TLR7-transfected eukaryotic cells.
• Specificity: Human TLR7.
  Species cross-reactivity: Dog
• Formulation: Purified: 100 μg in 200μl / 50 μg in 100 μl Tris-NaCl pH 8.
  Coupled: 100 μg in 200μl / 50 μg in 100 μl Tris PBS 50% glycerol.
  State: Purified
• Concentration: lot specific
• Conjugation: Unconjugated
• Storage: -20°C. KEEP CONTENTS STERILE: no preservative.
  Purified antibodies: avoid repeated freeze/thaw cycles.
  Coupled antibodies: glycerol protects from freezing.
• Gene Name: toll like receptor 7
• Database Link:
  Entrez Gene 51284 Human
  UniProt ID Q9NYK1
• Background: At least twelve members of the Toll family have been identified. TLR7 is expressed on plasmacytoid DCs, B and T lymphocytes, neutrophils, eosinophils, NK cells, and lung and placenta epithelial cells. TLR7 is localized to endosomal/lysosomal compartments and is reported to respond to single-stranded RNA. Plasmacytoid DCs express TLR7 and respond to activation by producing high amounts of IFN-a / b. TLR7 ligands are R848, Imiquimod (Imidazoquinolin family) and loxoribine (guanosin analog). (Diebold S.S.et al, Science 2004, 303, 1529; Barchet W.A. et al, 2005, Eur J Immunol 3:236; Lund J.M. et al., 2004, PNAS 5598; Hell F. et al., 2004 Science, 303, 1526 ; Gilliet M et al.,2008, Natur Rev Immunol.8, 594).The 66H3 clone was generated through mouse immunization with human TLR7-transfected eukaryotic cells, and was found to cross react with canine TLR7 (Bonnefont C, personal communication).
• Synonyms: Toll-like receptor 7