CD23 Mouse Monoclonal Antibody [Clone ID: 9P25]

CAT#: DDX0600P-100

CD23 mouse monoclonal antibody, clone 9P25

Specifications

Product Data

• Clone Name: 9P25
• Applications: FC, FN
• Recommended Dilution: Study of IgE binding to CD23 (Fc epsilon R2), FACS surface.
  
  Usage recommendation:
  *This monoclonal antibody may be used between 5-20 μg/ml.
  *Optimal dilution should be determined by each laboratory for each application.
  *Coupled antibody: to maintain RT before using.
  
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: EBV transformed lymphoblastoïd cell line.
• Formulation: 100 μg in 200μl / 50 μg in 100 μl Tris-NaCl pH 8
  State: Purified
• Concentration: 0.5 mg/ml
• Purification: QMA Hyper D ion exchange chromatography
• Background: CD23, also known as Fc epsilon RII, or FcεRII, is the "low affinity" receptor for IgE, an antibody isotype involved in allergy and resistance to parasites and is important in regulation of IgE levels. Human rIL-4 is able to induce the expression of low-affinity receptors for IgE (Fc epsilon RL/CD23) on resting B lymphocytes, as determined by the binding of either the anti Fc epsilon RL/CD23-specific mAb 25 or IgE. Stimulation of B cells with insolubilized anti-IgM antibody increases the number of cells expressing Fc epsilon RL/CD23 upon culturing with IL-4 and enhances the level of Fc epsilon RL/CD23 expression on these cells. Fc epsilon RL/CD23 induction is specific for IL-4 since IL-1 alpha, IL-2, IFN-gamma, B cell-derived B cell growth factor (BCGF), and a low-molecular-weight BCGF were ineffective. IFN-gamma strongly inhibited the induction of Fc epsilon RL/CD23 by IL-4. CD23 is a 45 kDa transmembrane glycoprotein associated with MHC Class II antigen, which belongs to the C-type lectin family. Expression of CD23 by B lymphocytes is up-regulated following mitogen or antigen activation. Soluble forms of CD23 are generated by proteolytic cleavage of the membrane molecule. (Defrance T. et al., J Exp Med, 1987,165, 1459 ; Bonnefoy J-Y., et al., 1996, Eur.Respir.J., 9, 635-665).
• Synonyms: Fc-epsilon-RII, FCER2, CD23A, FCE2, IGEBF