IL1 alpha (IL1A) Human Monoclonal Antibody [Clone ID: HuMab X3]

CAT#: DDX0710A546-100

IL1 alpha (IL1A) human monoclonal antibody, clone HuMab X3

Product Images

Inhibition of human IL-1α-induced IL-6 production by human synoviocytes by HuMab X3

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  Inhibition of human IL-1α-induced IL-6 production by human synoviocytes by HuMab X3 in the presence of either human IL-1β or IL-1α

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  "Cross-Reactivity of the HuMab X3“ using excess human IL-1β, IL-1Ra or IL-1α to protect against immunoprecipitation byHuMAb X3.

Specifications

Product Data

• Clone Name: HuMab X3
• Recommended Dilution: DDX0710P-50 / DDX0710P-100 Purified: Inhibition, Neutralization (Binding to IL1α and not to IL1 or IL1Ra, Neutralizing biological effects of natural and recombinant IL1α, Inhibition of IL1α binding to IL1 receptors.
  DDX0710A488-50 / DDX0710A488-100 Alexa-fluor®488 (on request).
  DDX0710A546-50 / DDX0710A546-100 Alexa-fluor®546 (on request).
  DDX0710A647-50 / DDX0710A647-100 Alexa-fluor®647 (on request).
  DDX0710B-50 / DDX0710B-100 Biotin (on request).
  
  Usage recommendation:
  *This monoclonal antibody may be used between 5-25 μg/ml.
  *Optimal dilution should be determined by each laboratory for each application.
  *Coupled antibody: to maintain RT before using.
  
• Reactivities: Human
• Host: Human
• Isotype: IgG4
• Clonality: Monoclonal
• Immunogen: CD40 activated and EBV immortalized human B lymphocytes.
• Specificity: Human IL1α (binds to Human IL1α but not to IL1β and IL1Ra).
• Formulation: Purified: 100 μg in 200μl / 50 μg in 100 μl Tris-NaCl pH 8.
  Coupled: 100 μg in 200μl / 50 μg in 100 μl Tris PBS 50% glycerol.
  Label: Alexa Fluor 546
• Concentration: lot specific
• Purification: QMA Hyper D ion exchange chromatography
• Conjugation: Alexa Fluor 546
• Storage: -20°C. KEEP CONTENTS STERILE: no preservative.
  Purified antibodies: avoid repeated freeze/thaw cycles.
  Coupled antibodies: glycerol protects from freezing.
• Gene Name: interleukin 1 alpha
• Database Link:
  Entrez Gene 3552 Human
  UniProt ID P01583
• Background: Interleukin-1 (IL-1) defines two polypeptides, IL-1α and IL-1β, that possess a wide spectrum of biological effects. Two natural antagonists of IL-1 action have been characterized: the IL-1 receptor antagonist (IL-1Ra) and a soluble form of the type II IL-1 receptor. Neutralizing autoantibodies to IL-1α have also been detected in sera of healthy individuals and patients with autoimmune or inflammatory diseases. By combining CD40-activation and Epstein-Barr virusimmortalization of B lymphocytes from individuals with circulating anti-IL-1α, a natural anti-IL- 1α monoclonal autoantibody was developed that bound specifically to human IL-1α, but not to IL- 1β and IL-1Ra, with a high affinity (Kd = 1.2 × 10⁻¹⁰ M).
  HuMAb X3 inhibited IL-1α binding to IL-1 receptors and neutralized biological activities of both recombinant and natural forms of IL-1α. This high affinity neutralizing anti-IL-1α human autoantibody can function as a third type of IL-1 antagonist. (Garrone P et al, Mol Immunol 1996, 33 (7-8), 649-58 ; Guillot-Chene P, Lebecque S, Rigal D, Annales Pharmaceutiques Françaises, 06/2009, 67 (3) 182-6).
• Synonyms: IL-1 alpha, IL1F1, IL1A, Hematopoietin-1