IL1 alpha (IL1A) Human Monoclonal Antibody [Clone ID: HuMab X3]

CAT#: DDX0710B-100

IL1 alpha (IL1A) human monoclonal antibody, clone HuMab X3


USD 680.00

2 Weeks*

Size
    • 100 ug

Product Images

Specifications

Product Data
Clone Name HuMab X3
Recommended Dilution DDX0710P-50 / DDX0710P-100 Purified: Inhibition, Neutralization (Binding to IL1α and not to IL1 or IL1Ra, Neutralizing biological effects of natural and recombinant IL1α, Inhibition of IL1α  binding to IL1 receptors.
DDX0710A488-50 / DDX0710A488-100 Alexa-fluor®488 (on request).

DDX0710A546-50 / DDX0710A546-100 Alexa-fluor®546 (on request).

DDX0710A647-50 / DDX0710A647-100 Alexa-fluor®647 (on request). 
DDX0710B-50 / DDX0710B-100 Biotin (on request).


Usage recommendation:

*This monoclonal antibody may be used between 5-25 μg/ml.
*Optimal dilution should be determined by each laboratory for each application.
*Coupled antibody: to maintain RT before using.
Reactivities Human
Host Human
Isotype IgG4
Clonality Monoclonal
Immunogen CD40 activated and EBV immortalized human B lymphocytes.
Specificity Human IL1α  (binds to Human IL1α but not to IL1β and IL1Ra).
Formulation Purified: 100 μg in 200μl / 50 μg in 100 μl Tris-NaCl pH 8.
Coupled: 100 μg in 200μl / 50 μg in 100 μl Tris PBS 50% glycerol.
Label: Biotin
Concentration lot specific
Purification QMA Hyper D ion exchange chromatography
Conjugation Biotin
Storage -20°C. KEEP CONTENTS STERILE: no preservative.
Purified antibodies: avoid repeated freeze/thaw cycles.
Coupled antibodies: glycerol protects from freezing.
Gene Name interleukin 1 alpha
Background Interleukin-1 (IL-1) defines two polypeptides, IL-1α and IL-1β, that possess a wide spectrum of biological effects. Two natural antagonists of IL-1 action have been characterized: the IL-1 receptor antagonist (IL-1Ra) and a soluble form of the type II IL-1 receptor. Neutralizing autoantibodies to IL-1α have also been detected in sera of healthy individuals and patients with autoimmune or inflammatory diseases. By combining CD40-activation and Epstein-Barr virusimmortalization of B lymphocytes from individuals with circulating anti-IL-1α, a natural anti-IL- 1α monoclonal autoantibody was developed that bound specifically to human IL-1α, but not to IL- 1β and IL-1Ra, with a high affinity (Kd = 1.2 × 10−10 M).
HuMAb X3 inhibited IL-1α binding to IL-1 receptors and neutralized biological activities of both recombinant and natural forms of IL-1α. This high affinity neutralizing anti-IL-1α human autoantibody can function as a third type of IL-1 antagonist. (Garrone P et al, Mol Immunol 1996, 33 (7-8), 649-58 ; Guillot-Chene P, Lebecque S, Rigal D, Annales Pharmaceutiques Françaises, 06/2009, 67 (3) 182-6).
Synonyms IL-1 alpha, IL1F1, IL1A, Hematopoietin-1
Reference Data

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.