alpha Synuclein (SNCA) Mouse Monoclonal Antibody [Clone ID: AS11]

CAT#: DDX0910A546-100

alpha Synuclein (SNCA) mouse monoclonal antibody, clone AS11

Product Images

IF staining of human neuroblastoma (SH-SY5Y) expressing endogenous α-synuclein

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  IHC staining of pathological human brainswith AS11

Specifications

Product Data

• Clone Name: AS11
• Applications: IF
• Recommended Dilution: DDX0910P-50 / DDX0910P-100 Purified: ImmunoFluorescence, ImmunoHistoChemistry frozen sections, Western Blot.
  DDX0910A488-50 / DDX0910A488-100 Alexa-fluor®488: ImmunoFluorescence.
  DDX0910A546-50 / DDX0910A546-100 Alexa-fluor®546: ImmunoFluorescence.
  DDX0910A647-50 / DDX0910A647-100 Alexa-fluor®647: ImmunoFluorescence.
  DDX0910HRP-50 / DDX0910HRP-100 Horseradish Peroxydase: ImmunoHistoChemistry frozen sections, Western Blot.
  
  Usage recommendation:
  *This monoclonal antibody may be used between 5-20 μg/ml.
  *Optimal dilution should be determined by each laboratory for each application.
  *Coupled antibody: to maintain RT before use.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG
• Clonality: Monoclonal
• Immunogen: rhu α-synuclein (SIGMA).
• Specificity: N-terminal portion of Human α-synuclein (aa1-60)
  Species cross-reactivity: Negative for Murine α-synuclein.
• Formulation: Purified: 100 μg in 200μl / 50 μg in 100 μl Tris-NaCl pH 8.
  Coupled: 100 μg in 200μl / 50 μg in 100 μl Tris PBS 50% glycerol.
  Label: Alexa Fluor 546
• Concentration: lot specific
• Purification: QMA Hyper D ion exchange chromatography
• Conjugation: Alexa Fluor 546
• Storage: -20°C. KEEP CONTENTS STERILE: no preservative.
  Purified antibodies: avoid repeated freeze/thaw cycles.
  Coupled antibodies: glycerol protects from freezing.
• Gene Name: synuclein alpha
• Database Link:
  Entrez Gene 6622 Human
  UniProt ID P37840
• Background: α-synuclein (snca) is a140 aas neuronal protein predominantly present in synaptic junctions, specially studied for its relevance in understanding sporadic and familial forms of Parkinson’s disease and related neurodegenerative disorders. In an attempt to break the immunotolerance since the amino-acid sequence is highly conserved between species (95.3% homology between human and mouse), we have immunized C57BL/6 inbred mice presenting a natural deletion for snca with recombinant human α-synuclein and obtained a murine mAb (AS11) recognizing endogenous human α-synuclein. (Kahle et al.,2000, J Neurosci 2000;20:6365–73 ; Specht and Schoepfer, BMC Neurosci 2001;2:11 ; Mougenot AL et al, J Neurosci Methods. 2010 Oct 15;192(2):268-76).
• Synonyms: NACP, PARK1