HIV-1 (gp41) Mouse Monoclonal Antibody [Clone ID: 425D11.01]

CAT#: DDX1306A488-100

HIV-1 (gp41) mouse monoclonal antibody, clone 425D11.01

Product Images

FACS staining of HEK293-gp41TM cell line (Dawood R et al, 2013)

• 

  Inhibition of HR1/HR2 fusion complex formation

Specifications

Product Data

• Clone Name: 425D11.01
• Applications: ELISA, FC, IF, NEUT
• Recommended Dilution: DDX1306P-50 / DDX1306P-100 Purified: Blocking of HR1/HR2 complex formation, Cell-coated ELISA, FACS surface, ImmunoFluorescence, Neutralization, Inhibition.
  DDX1306A488-50 / DDX1306A488-100 Alexa- fluor®A488 (on request): Blocking of HR1/HR2 complex formation, Cell-coated ELISA, FACS surface, ImmunoFluorescence, Neutralization, Inhibition.
  DDX1306A546-50 / DDX1306A546-100 Alexa- fluor®546 (on request): Blocking of HR1/HR2 complex formation, Cell-coated ELISA, FACS surface, ImmunoFluorescence, Neutralization, Inhibition.
  DDX1306A647-50 / DDX1306A647-100 Alexa- fluor®647 (on request): Blocking of HR1/HR2 complex formation, Cell-coated ELISA, FACS surface, ImmunoFluorescence, Neutralization, Inhibition.
  DDX1306B-50 / DDX1306B-100 Biotin (on request): Blocking of HR1/HR2 complex formation, Cell-coated ELISA, FACS surface, ImmunoFluorescence, Neutralization, Inhibition.
  
  Applications tested: Dawood R et al, AIDS. 27(5):717-730, 2013.
  Usage recommendation:
  *This monoclonal antibody may be used between 5-25 μg/ml.
  *Optimal dilution should be determined by each laboratory for each application.
  *Coupled antibody: to maintain RT before using.
  
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: HR1-PID-HR2-transfected HEK 293 cells (HIV-1, 92BR025, Clade C).
• Specificity: Conformational epitope formed by aa 531-560 (HR1) and 623-661 (HR2).
  Clone 425D11.01 referred to as clone 3 in the article.
• Formulation: Purified: 100 μg in 200μl / 50 μg in 100 μl Tris-NaCl pH 8.
  Coupled: 100 μg in 200μl / 50 μg in 100 μl Tris PBS 50% glycerol.
  Label: Alexa Fluor 488
• Concentration: 0.5 mg/ml
• Purification: QMA Hyper D ion exchange chromatography
• Conjugation: Alexa Fluor 488
• Background: HIV-1-gp41 is a subunit of the envelope protein complex of HIV. HIV-1-gp41 is responsible for the fusion between the viral and the cell membranes and thus represents a target for HIV vaccines. Trimeric Env is composed of gp120, which is non-covalently associated with the membrane-anchored fusion protein gp41. HIV-1 gp120 binding to CD4 and co-receptor (CCR5 or CXCR4) induces conformational changes, resulting in gp41 exposure and in the production of fusion-intermediate conformation of gp41 (HR1 and HR2). 3 neutralizing anti-gp41 monoclonal antibodies were selected from mice immunized with 293T cells stably transfected with a construct expressing HR1 plus HR2. (Dawood R et al, AIDS. 27(5):717-730, 2013)
• Synonyms: HIV1, HIV-I, Human immunodeficiency virus type 1